Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)
![Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)](https://www.abcam.com/ps/products/32/ab32203/Images/ab32203-270700-anti-rsk1-p90-phospho-s380-antibody-e239-western-blot.jpg "Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E239] to RSK1 p90 (phospho S380)
- Suitable for: Dot blot, WB, ICC/IF, IP
- Reacts with: Human
Overview
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Product name
Anti-RSK1 p90 (phospho S380) antibody [E239]
See all RSK1 p90 primary antibodies -
Description
Rabbit monoclonal [E239] to RSK1 p90 (phospho S380) -
Host species
Rabbit -
Specificity
The antibody only detects RSK1 phosphorylated on Serine 380. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIP HumanWB Human
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Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Ser380 of human p90RSK1.
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Positive control
- A431 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
E239 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)All lanes : Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203) at 1/5000 dilution
Lane 1 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates
Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) treated with epidermal growth factor at 100ng/ml for 10 min. Whole cell lysates.
Lane 3 : A431 (Human epidermoid carcinoma epithelial cell) treated with epidermal growth factor at 100ng/ml for 10 min. Whole cell lysates. Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 83 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)](https://www.abcam.com/ps/products/32/ab32203/Images/ab32203-270686-anti-rsk1-p90-phospho-s380-antibody-e239-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized A431(Human epidermoid carcinoma cell line) cells, treated and non-treated, labeling RSK1 p90 (phospho S380) with Ab32203 at 1/200 dilution followed by Goat anti-Rabbit secondary IgG AlexaFluor®488 (ab150077) secondary antibody at 1/1000 dilution (green).
The green staining was increased in the EGF(ab9697 100ng/ml, 10min) treated A431 cells when compared with A431 cells without treatment. After LP treatment, the green signaling was obviously decreased.
For the pan antibody, there was no great difference after EGF (100ng/ml, 10min) or EGF (100ng/ml, 10min) + LP treatment. The data showed mostly Cytoplasm staining.
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![Immunoprecipitation - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)](https://www.abcam.com/ps/products/32/ab32203/Images/ab32203-271756-anti-rsk1-p90-phospho-s380-antibody-e239-immunoprecipitation.jpg)
Immunoprecipitation - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)RSK1 p90 (phospho S380) was immunoprecipitated from 10μg A431 (human epidermoid carcinoma) whole cell lysate with ab32203 at 1/20 dilution (0.5μg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab32203 at 1/200 dilution (0.54 μg/ml). VeriBlot for IP Detection Reagent (HRP) ab131366, was used for detection at 1/1000 dilution.
Lane 1 Input: A431(human epidermoid carcinoma) treated with 100ng/ml EGF for 10min whole cell lysate 10μg
Lane 2 (+): ab32203 + A431(human epidermoid carcinoma) treated with 100ng/ml EGF for 10min whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32203 in A431(human epidermoid carcinoma) treated with 100ng/ml EGF for 10min whole cell lysateBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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![Dot Blot - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)](https://www.abcam.com/ps/products/32/ab32203/Images/ab32203-271124-anti-rsk1-p90-phospho-s380-antibody-e239-dot-blot.jpg)
Dot Blot - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)Dot blot analysis of RSK1 p90 (phospho S380) phospho peptide (Lane 1) and RSK1 p90 non-phospho peptide (Lane 2) labeling RSK1 p90 (phospho S380) with ab32203 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)](https://www.abcam.com/ps/products/32/ab32203/Images/ab32203-7-benefits-of-recombinant-antibodies.png)
Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)
