Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] - BSA and Azide free (ab171376)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E238] to RSK1 p90 (phospho T359 + S363) - BSA and Azide free
  • Suitable for: Dot blot, WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] - BSA and Azide free
    See all RSK1 p90 primary antibodies

  • Description

    Rabbit monoclonal [E238] to RSK1 p90 (phospho T359 + S363) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, WB, IP, IHC-Pmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: NIH/3T3 whole cell lysate.

  • General notes

    Ab171376 is the carrier-free version of ab32413. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab171376 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] - BSA and Azide free (ab171376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] - BSA and Azide free (ab171376)

    Ab32413, at a 1/100 dilution, staining RSK1 p90 in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.
    Left image : Untreated.
    Right image : Treated with Phosphatase.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32413).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] - BSA and Azide free (ab171376)
    Immunoprecipitation - Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] - BSA and Azide free (ab171376)

    Purified ab32413 at 1/100 dilution (2µg) immunoprecipitating RSK1 p90 in NIH/3T3 whole cell lysate.
    Lane 1 (input): NIH/3T3 (Mouse embryonic fibroblast) serum starved for 4h, then treated with 20% FBS for 30min whole cell lysate 10µg
    Lane 2 (+): ab32413 + NIH/3T3 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab171376 in NIH/3T3 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 90 kDa
    Lower bands could be cleavage product. (PMID: 29290690)
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32413).

  • Dot Blot - Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] - BSA and Azide free (ab171376)
    Dot Blot - Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] - BSA and Azide free (ab171376)

    Dot blot analysis of RSK1 p90 (phospho T359 + S363) phospho peptide (Lane 1), RSK1 p90 phospho T359 phospho peptide (Lane 2), RSK1 p90 phospho S36 phospho peptide (Lane 3) and RSK1 p90 non-phospho peptide (Lane 4) labeling RSK1 p90 (phospho T359 + S363) phospho peptide with purified ab32413 at a dilution of 1/1000 dilution (2.056ug/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution.

    Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST .

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32413).

  • Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] - BSA and Azide free (ab171376)
    Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] - BSA and Azide free (ab171376)

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