Anti-MAPK6/ERK3 (phospho S189) antibody (ab74032)
Key features and details
- Rabbit polyclonal to MAPK6/ERK3 (phospho S189)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MAPK6/ERK3 (phospho S189) antibody
See all MAPK6/ERK3 primary antibodies -
Description
Rabbit polyclonal to MAPK6/ERK3 (phospho S189) -
Host species
Rabbit -
Specificity
ab74032 detects endogenous levels of MAPK6/ERK3 only when phosphorylated at serine 189.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Synthetic peptide corresponding to Human MAPK6/ERK3 (phospho S189).
Sequence:H-L-Sp-E-G
Database link: Q16659 -
General notes
Previously labelled as MAPK6.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab74032 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 (phospho S189) antibody (ab74032)Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ab74032, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising phosphopeptide.
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Western blot - Anti-MAPK6/ERK3 (phospho S189) antibody (ab74032)All lanes : Anti-MAPK6/ERK3 (phospho S189) antibody (ab74032) at 1/500 dilution
Lane 1 : Extracts from mouse brain
Lane 2 : Extracts from mouse brain with immunising phosphopeptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 83 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
