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Human ELK1 knockout HeLa cell pellet (ab278861)

Overview

  • Product name

    Human ELK1 knockout HeLa cell pellet
    See all ELK1 kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HeLa

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, Homozygous: 222 bp insertion in exon 2.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.

  • Tissue specificity

    Lung and testis.

  • Sequence similarities

    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.

  • Post-translational
    modifications

    Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
    On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Alternative names

    • ELK 1
    • Elk1
    • ELK1 member of ETS oncogene family
    • ELK1 protein
    • ELK1, ETS transcription factor
    • ELK1_HUMAN
    • ELK2 member of ETS oncogene family
    • ETS domain containing protein Elk 1
    • ETS domain containing protein Elk1
    • ETS domain protein Elk1
    • ETS domain-containing protein Elk-1
    • ETS like gene 1
    • Member of ETS oncogene family
    • Oncogene Elk1
    • Tyrosine kinase (ELK1) oncogene
    see all

Properties

Images

  • Western blot - Human ELK1 knockout HeLa cell pellet (ab278861)
    Western blot - Human ELK1 knockout HeLa cell pellet (ab278861)
    Lane 1: Wild-type HeLa cell lysate (20µg)

    Lane 2: ELK1 knockout HeLa cell lysate (20µg)
    Lanes 1- 2: Merged signal (red and green). Green - ab188316 observed at 55 kDa. Red - loading control ab8245 observed at 37 kDa.
    ab188316 Anti-ELK1 antibody [EPR1913(2)] was shown to specifically react with ELK1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261764 (knockout cell lysate ab256904) was used. Wild-type and ELK1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab188316 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
  • Western blot - Human ELK1 knockout HeLa cell pellet (ab278861)
    Western blot - Human ELK1 knockout HeLa cell pellet (ab278861)

    Lane 1: Wild-type HeLa cell lysate (20µg)
    Lane 2: ELK1 knockout HeLa cell lysate (20µg)
    Lanes 1- 2: Merged signal (red and green). Green - ab32106 observed at 55 kDa. Red - loading control ab8245 observed at 37 kDa.
    ab32106 Anti-ELK1 antibody [E277] - ChIP Grade was shown to specifically react with ELK1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261764 (knockout cell lysate ab256904) was used. Wild-type and ELK1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32106 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human ELK1 knockout HeLa cell pellet (ab278861)
    Sanger Sequencing - Human ELK1 knockout HeLa cell pellet (ab278861)
    Homozygous: 222 bp insertion in exon 2

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