Antibodies, Proteins, Kits and Reagents for Life Science

324 988 ₸ Product size

100 µl 324 988 ₸

Order now and get it on Tuesday March 02, 2021

Anti-p38 (phospho T180 + Y182) antibody [EPR18120] (ab195049)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18120] to p38 (phospho T180 + Y182)
Suitable for: WB, Dot blot
Reacts with: Mouse, Human

Product name

Anti-p38 (phospho T180 + Y182) antibody [EPR18120]
See all p38 primary antibodies
Description

Rabbit monoclonal [EPR18120] to p38 (phospho T180 + Y182)
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293 whole cell lysate treated with UV (60 J/M2) for 60 minutes; NIH/3T3 whole cell lysate treated with UV (60 J/M2) for 60 minutes.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18120
Isotype

IgG
Research areas

Dot Blot - Anti-p38 (phospho T180 + Y182) antibody [EPR18120] (ab195049)

Dot blot analysis of p38 (phospho T180) peptide (Lane 1), p38 (phospho Y182) (Lane 2), p38 (phospho T180 + Y182) peptide (Lane 3), and non-phospho peptide (Lane 4), labeled using ab195049 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody (ab97051) at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Western blot - Anti-p38 (phospho T180 + Y182) antibody [EPR18120] (ab195049)

All lanes : Anti-p38 (phospho T180 + Y182) antibody [EPR18120] (ab195049) at 1/1000 dilution

Lane 1 : Untreated HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysates
Lane 2 : HEK-293 whole cell lysates treated with UV (60 J/M2) for 60 minutes

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

Predicted band size: 41 kDa
Observed band size: 41 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-p38 (phospho T180 + Y182) antibody [EPR18120] (ab195049)

All lanes : Anti-p38 (phospho T180 + Y182) antibody [EPR18120] (ab195049) at 1/1000 dilution

Lane 1 : Untreated NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lane 2 : NIH/3T3 whole cell lysate treated with UV (60 J/M2) for 60 minutes

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

Predicted band size: 41 kDa
Observed band size: 41 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-p38 (phospho T180 + Y182) antibody [EPR18120] (ab195049)

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