Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16587] to p38 (phospho T180)
  • Suitable for: Dot blot, WB, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-p38 (phospho T180) antibody [EPR16587]
    See all p38 primary antibodies

  • Description

    Rabbit monoclonal [EPR16587] to p38 (phospho T180)

  • Host species

    Rabbit

  • Specificity

    ab178867 also recognises phosphorylated Y182 site.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa treated with anisomycin whole cell lysates. IHC-P: Human squamous cell cervical carcinoma and transitional cell carcinoma of bladder tissues. Flow Cyt: Jurkat cells treated with anisomycin.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
    Western blot - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
    Lane 1 : Anti-p38 (phospho T180) antibody [EPR16587] (ab178867) at 1/1000 dilution
    Lane 2 : Anti-p38 (phospho T180) antibody [EPR16587] (ab178867) at 1/10000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates treated with 250ng/ml anisomycin for 30 minutes
    Lane 2 : Untreated Hela whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
    Dot Blot - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)

    Dot blot analysis of p38 peptides using ab178867 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated secondary antibody at 1/1000 dilution. Blocking and diluting buffer was 5% NFDM/TBST.

    Lane 1: Single phospho peptide pT180
    Lane 2: Single phospho peptide pY182
    Lane 3: Double phospho peptide pT180/pY182
    Lane 4: Non-phospho peptide

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)

    Immunohistochemical analysis of paraffin-embedded Human squamous cell cervical carcinoma tissue labeling p38 (phospho T180) with ab178867 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on cancer cells of cervix is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling p38 (phospho T180) with ab178867 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear with additional cytoplasmic staining on cancer cells of bladder is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
    Flow Cytometry - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)

    Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells treated with Anisomycin (250ng/ml for 30min) labeling p38 (phospho T180) with ab178867 at 1/340 dilution followed by Goat anti rabbit IgG (FITC) at 1/150 (red).

    Controls:-  

    Blue - Unlabelled (Cells without incubation with primary antibody and secondary antibody)

    Black - Rabbit monoclonal IgG Isotype control

    Orange - Jurkat cells treated with Anisomycin (250ng/ml for 30min) labeling p38 (phospho T180) with ab178867 at 1/340 preincubated with 1mg/ml p38 (phospho T180) peptide

  • Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
    Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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