Antibodies, Proteins, Kits and Reagents for Life Science

348 441 ₸ Product size

100 µl 348 441 ₸

Order now and get it on Tuesday March 09, 2021

Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16587] to p38 (phospho T180)
Suitable for: Dot blot, WB, Flow Cyt, IHC-P
Reacts with: Human

Product name

Anti-p38 (phospho T180) antibody [EPR16587]
See all p38 primary antibodies
Description

Rabbit monoclonal [EPR16587] to p38 (phospho T180)
Host species

Rabbit
Specificity

ab178867 also recognises phosphorylated Y182 site.

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa treated with anisomycin whole cell lysates. IHC-P: Human squamous cell cervical carcinoma and transitional cell carcinoma of bladder tissues. Flow Cyt: Jurkat cells treated with anisomycin.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16587
Isotype

IgG
Research areas

Western blot - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)

Lane 1 : Anti-p38 (phospho T180) antibody [EPR16587] (ab178867) at 1/1000 dilution
Lane 2 : Anti-p38 (phospho T180) antibody [EPR16587] (ab178867) at 1/10000 dilution

Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates treated with 250ng/ml anisomycin for 30 minutes
Lane 2 : Untreated Hela whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa
Observed band size: 41 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Dot Blot - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)

Dot blot analysis of p38 peptides using ab178867 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated secondary antibody at 1/1000 dilution. Blocking and diluting buffer was 5% NFDM/TBST.

Lane 1: Single phospho peptide pT180
Lane 2: Single phospho peptide pY182
Lane 3: Double phospho peptide pT180/pY182
Lane 4: Non-phospho peptide
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)

Immunohistochemical analysis of paraffin-embedded Human squamous cell cervical carcinoma tissue labeling p38 (phospho T180) with ab178867 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on cancer cells of cervix is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)

Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling p38 (phospho T180) with ab178867 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear with additional cytoplasmic staining on cancer cells of bladder is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)

Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells treated with Anisomycin (250ng/ml for 30min) labeling p38 (phospho T180) with ab178867 at 1/340 dilution followed by Goat anti rabbit IgG (FITC) at 1/150 (red).
Controls:-
Blue - Unlabelled (Cells without incubation with primary antibody and secondary antibody)
Black - Rabbit monoclonal IgG Isotype control
Orange - Jurkat cells treated with Anisomycin (250ng/ml for 30min) labeling p38 (phospho T180) with ab178867 at 1/340 preincubated with 1mg/ml p38 (phospho T180) peptide
Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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