Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p38 alpha/MAPK14 antibody [E229] - BSA and Azide free (ab225534)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E229] to p38 alpha/MAPK14 - BSA and Azide free
  • Suitable for: WB, IP, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-p38 alpha/MAPK14 antibody [E229] - BSA and Azide free
    See all p38 alpha/MAPK14 primary antibodies

  • Description

    Rabbit monoclonal [E229] to p38 alpha/MAPK14 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, C6, NIH/3T3 or HeLa whole cell lysate (ab150035). ICC/IF: NIH/3T3 cell lysate.

  • General notes

    Ab225534 is the carrier-free version of ab170099. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab225534 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-p38 alpha/MAPK14 antibody [E229] - BSA and Azide free (ab225534)
    Western blot - Anti-p38 alpha/MAPK14 antibody [E229] - BSA and Azide free (ab225534)

    This WB data was generated using the same anti-p38 antibody clone [E229] in a different buffer formulation (cat# ab170099).

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: p38 knockout HAP1 cell lysate (20 µg) 
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg) 
    Lanes 1 - 4: Merged signal (red and green). Green - ab170099 observed at 40 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab170099 was shown to specifically react with p38 when p38 knockout samples were used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab170099 and

    ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-p38 alpha/MAPK14 antibody [E229] - BSA and Azide free (ab225534)
    Immunocytochemistry/ Immunofluorescence - Anti-p38 alpha/MAPK14 antibody [E229] - BSA and Azide free (ab225534)
    Immunocytochemistry/ Immunofluorescence analysis of HeLa(Human epithelial cell line from cervix adenocarcinoma) cells labeling p38 with ab170099 at 1/250. Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077, an Alexa Fluor® 488 Goat anti-Rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889, an anti-alpha tubulin antibody [DM1A] microtubule marker (Alexa Fluor® 594) at 1/200. Nuclei counterstained with DAPI (blue).

    Confocal image shows nuclear and cytoplasmic staining on HeLa cell line.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170099).

  • Immunoprecipitation - Anti-p38 alpha/MAPK14 antibody [E229] - BSA and Azide free (ab225534)
    Immunoprecipitation - Anti-p38 alpha/MAPK14 antibody [E229] - BSA and Azide free (ab225534)

    ab170099 (purified) at 1/20 immunoprecipitating p38 in Jurkat whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) was used for detection at 1/1,500 dilution. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170099).

  • Flow Cytometry - Anti-p38 alpha/MAPK14 antibody [E229] - BSA and Azide free (ab225534)
    Flow Cytometry - Anti-p38 alpha/MAPK14 antibody [E229] - BSA and Azide free (ab225534)
    Flow Cytometry analysis of HeLa cells labelling p38 with purified ab170099 at 1/40 (red). Cells were fixed with 4% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170099).

  • Anti-p38 alpha/MAPK14 antibody [E229] - BSA and Azide free (ab225534)
    Anti-p38 alpha/MAPK14 antibody [E229] - BSA and Azide free (ab225534)

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