Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] - BSA and Azide free (ab227995)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y122] to p38 beta/MAPK11 + p38 alpha/MAPK14 - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] - BSA and Azide free

  • Description

    Rabbit monoclonal [Y122] to p38 beta/MAPK11 + p38 alpha/MAPK14 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, Jurkat, K562, HEK-293T and MCF7 cell lysates. IHC-P: Human skin carcinoma. ICC/IF: MCF7 cells.

  • General notes

    Ab227995 is the carrier-free version of ab32142. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab227995 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] - BSA and Azide free (ab227995)
    Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] - BSA and Azide free (ab227995)
    All lanes : Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142) at 1/1000 dilution

    Lane 1 : Recombinant Human p38 beta/MAPK11 protein (ab117219)
    Lane 2 : Recombinant Human p38 gamma/MAPK12 protein (ab117221)
    Lane 3 : Recombinant Human p38 delta/MAPK13 protein (ab113869)
    Lane 4 : Recombinant human p38 alpha/MAPK14 protein (ab82188)

    Predicted band size: 41 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab32142).

     
  • Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] - BSA and Azide free (ab227995)
    Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] - BSA and Azide free (ab227995)
    All lanes : Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Wild-type HEK-293T cell lysate
    Lane 4 : MAPK14 knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 41 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab32142).

    Lanes 1 - 4: Merged signal (red and green). Green - ab32142 observed at 40 kDa. Red - loading control, ab130007 observed at 125 kDa.  

     ab32142 was shown to react with p38 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255406 (knockout cell lysate ab263787) was used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab32142 and Anti-Vinculin antibody [VIN-54] (ab130007) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] - BSA and Azide free (ab227995)
    Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] - BSA and Azide free (ab227995)
    All lanes : Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : p38 knockout HAP1 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 41 kDa



    This WB data was generated using the same anti-p38 antibody clone, Y122, in a different buffer formulation (cat# ab32142).


    Lanes 1 - 4: Merged signal (red and green). Green - ab32142 observed at 40 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab32142 was shown to specifically react with p38 when p38 knockout samples were used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab32142 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)  secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] - BSA and Azide free (ab227995)
    Immunocytochemistry/ Immunofluorescence - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] - BSA and Azide free (ab227995)

    Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling p38 (green) with purified ab32142 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32142).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] - BSA and Azide free (ab227995)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] - BSA and Azide free (ab227995)

    This IHC data was generated using the same anti-p38 antibody clone, Y122, in a different buffer formulation (cat# ab32142).

    Ab32142, at a 1/100 dilution, staining p38 in paraffin embedded human skin carcinoma tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] - BSA and Azide free (ab227995)
    Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] - BSA and Azide free (ab227995)

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