Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p38 (phospho T180) antibody [EPR16587] - BSA and Azide free (ab236527)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16587] to p38 (phospho T180) - BSA and Azide free
  • Suitable for: WB, Dot blot, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-p38 (phospho T180) antibody [EPR16587] - BSA and Azide free
    See all p38 primary antibodies

  • Description

    Rabbit monoclonal [EPR16587] to p38 (phospho T180) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Dot blot, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human squamous cell cervical carcinoma tissue.

  • General notes

    Ab236527 is the carrier-free version of ab178867. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236527 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-p38 (phospho T180) antibody [EPR16587] - BSA and Azide free (ab236527)
    Flow Cytometry - Anti-p38 (phospho T180) antibody [EPR16587] - BSA and Azide free (ab236527)

    Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells treated with Anisomycin (250ng/ml for 30min) labeling p38 (phospho T180) with ab178867 at 1/340 dilution followed by Goat anti rabbit IgG (FITC) at 1/150 (red).

    Controls:-  

    Blue - Unlabelled (Cells without incubation with primary antibody and secondary antibody)

    Black - Rabbit monoclonal IgG Isotype control

    Orange - Jurkat cells treated with Anisomycin (250ng/ml for 30min) labeling p38 (phospho T180) with ab178867 at 1/340 preincubated with 1mg/ml p38 (phospho T180) peptide

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178867).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180) antibody [EPR16587] - BSA and Azide free (ab236527)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180) antibody [EPR16587] - BSA and Azide free (ab236527)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling p38 (phospho T180) with ab178867 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear with additional cytoplasmic staining on cancer cells of bladder is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178867).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Dot Blot - Anti-p38 (phospho T180) antibody [EPR16587] - BSA and Azide free (ab236527)
    Dot Blot - Anti-p38 (phospho T180) antibody [EPR16587] - BSA and Azide free (ab236527)

    Dot blot analysis of p38 peptides using ab178867 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated secondary antibody at 1/1000 dilution. Blocking and diluting buffer was 5% NFDM/TBST.

    Lane 1: Single phospho peptide pT180
    Lane 2: Single phospho peptide pY182
    Lane 3: Double phospho peptide pT180/pY182
    Lane 4: Non-phospho peptide

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178867).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180) antibody [EPR16587] - BSA and Azide free (ab236527)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180) antibody [EPR16587] - BSA and Azide free (ab236527)

    Immunohistochemical analysis of paraffin-embedded Human squamous cell cervical carcinoma tissue labeling p38 (phospho T180) with ab178867 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on cancer cells of cervix is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178867).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-p38 (phospho T180) antibody [EPR16587] - BSA and Azide free (ab236527)
    Anti-p38 (phospho T180) antibody [EPR16587] - BSA and Azide free (ab236527)

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