Antibodies, Proteins, Kits and Reagents for Life Science

300 865 ₸ Product size

100 µl 300 865 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ELK1 antibody [EPR1913(2)] (ab188316)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1913(2)] to ELK1
Suitable for: WB, ICC/IF
Knockout validated
Reacts with: Human

Product name

Anti-ELK1 antibody [EPR1913(2)]
See all ELK1 primary antibodies
Description

Rabbit monoclonal [EPR1913(2)] to ELK1
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa and K562 cell lysate. ICC: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR1913(2)
Isotype

IgG
Research areas

Western blot - Anti-ELK1 antibody [EPR1913(2)] (ab188316)

All lanes : Anti-ELK1 antibody [EPR1913(2)] (ab188316) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ELK1 knockout HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 45 kDa
Observed band size: 55 kDa
why is the actual band size different from the predicted?

Lanes 1- 2: Merged signal (red and green). Green - ab188316 observed at 55 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab188316 was shown to react with ELK1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261764 (knockout cell lysate ab256904) was used. Wild-type HeLa and ELK1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab188316 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-ELK1 antibody [EPR1913(2)] (ab188316)

Immunocytochemical analysis of HeLa cells fixed in 2% paraformaldehyde labeling ELK1 with ab188316 at 1/100 dilution and Goat anti rabbit IgG(Alexa Fluor® 488) at 1/200 dilution. Counterstained with DAPI (blue).
Western blot - Anti-ELK1 antibody [EPR1913(2)] (ab188316)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: ELK1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa positve whole cell lysate (20 µg)
Lane 4: MCF7 negative whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab188316 observed at 55 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab188316 was shown to specifically react with ELK1 in wild-type HAP1 cells as signal was lost in ELK1 knockout cells. Wild-type and ELK1 knockout samples were subjected to SDS-PAGE. Ab188316 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-ELK1 antibody [EPR1913(2)] (ab188316)

All lanes : Anti-ELK1 antibody [EPR1913(2)] (ab188316) at 1/1000 dilution

Lane 1 : Hela cell lysate
Lane 2 : K562 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa
Anti-ELK1 antibody [EPR1913(2)] (ab188316)

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