Anti-ELK1 antibody (ab125085)
Key features and details
- Rabbit polyclonal to ELK1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ELK1 antibody
See all ELK1 primary antibodies -
Description
Rabbit polyclonal to ELK1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to between amino acids 378-428 of Human ELK1 (NP_005220.2).
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Positive control
- 293T and HeLa whole cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab125085 is affinity purified using an epitope specific to ELK1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ELK1 antibody (ab125085)All lanes : Anti-ELK1 antibody (ab125085) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-ELK1 antibody (ab125085)Detection of ELK1 by Western Blot of Immunprecipitate.
ab125085 at 1 µg/ml staining ELK1 in 293T whole cell lysate immunoprecipitated using ab125085 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
