Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PCK1/PEPC antibody (ab87340)

Key features and details

  • Rabbit polyclonal to PCK1/PEPC
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-PCK1/PEPC antibody
    See all PCK1/PEPC primary antibodies

  • Description

    Rabbit polyclonal to PCK1/PEPC

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human PCK1/PEPC aa 200-300 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab217264)

  • Positive control

    • Recombinant Human PCK1/PEPC protein (ab114362) can be used as a positive control in WB. This antibody gave a positive signal in the following tissue lysates: Human Liver Rat Liver Mouse Liver

  • General notes

     This product was previously labelled as PCK1

     

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab87340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Rat
All applications
Sheep
Horse
Chicken
Cow
Pig
Zebrafish
Orangutan
Application Abreviews Notes
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
Notes
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).

Target

  • Function

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

  • Tissue specificity

    Major sites of expression are liver, kidney and adipocytes.

  • Pathway

    Carbohydrate biosynthesis; gluconeogenesis.

  • Involvement in disease

    Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.

  • Sequence similarities

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

  • Post-translational
    modifications

    Acetylation is increased on addition of glucose and appears to regulate the protein stability.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • cytosolic [GTP] antibody
    • GTP antibody
    • PCK1 antibody
    • PCKGC_HUMAN antibody
    • PEP carboxykinase antibody
    • PEPCK-C antibody
    • PEPCK1 antibody
    • PEPCKC antibody
    • Phosphoenolpyruvate carboxykinase 1 (soluble) antibody
    • Phosphoenolpyruvate carboxykinase 1 antibody
    • Phosphoenolpyruvate carboxykinase antibody
    • Phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody
    • Phosphoenolpyruvate carboxykinase, cytosolic antibody
    • Phosphoenolpyruvate carboxylase antibody
    • Phosphopyruvate carboxylase antibody
    see all

Images

  • Western blot - Anti-PCK1/PEPC antibody (ab87340)
    Western blot - Anti-PCK1/PEPC antibody (ab87340)
    All lanes : Anti-PCK1/PEPC antibody (ab87340) at 2 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Liver (Rat) Tissue Lysate
    Lane 3 : Liver (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 70 kDa
    Observed band size: 70 kDa


    Exposure time: 15 minutes

References (2)

Publishing research using ab87340? Please let us know so that we can cite the reference in this datasheet.

ab87340 has been referenced in 2 publications.

  • Ma X  et al. Mechanism of continuous high temperature affecting growth performance, meat quality, and muscle biochemical properties of finishing pigs. Genes Nutr 14:23 (2019). PubMed: 31367261
  • Wang HY  et al. AS160 deficiency causes whole-body insulin resistance via composite effects in multiple tissues. Biochem J 449:479-89 (2013). PubMed: 23078342

Images

  • Western blot - Anti-PCK1/PEPC antibody (ab87340)
    Western blot - Anti-PCK1/PEPC antibody (ab87340)
    All lanes : Anti-PCK1/PEPC antibody (ab87340) at 2 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Liver (Rat) Tissue Lysate
    Lane 3 : Liver (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 70 kDa
    Observed band size: 70 kDa


    Exposure time: 15 minutes

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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