Anti-PCK1/PEPC antibody (ab70358)
Key features and details
- Rabbit polyclonal to PCK1/PEPC
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PCK1/PEPC antibody
See all PCK1/PEPC primary antibodies -
Description
Rabbit polyclonal to PCK1/PEPC -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide corresponding to Human PCK1/PEPC aa 550 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab86626) -
Positive control
- Recombinant Human PCK1/PEPC protein (ab119469) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: HepG2 Whole Cell Lysate Rat Liver Tissue Lysate Mouse Liver Tissue Lysate Human Liver Tissue Lysate - adult normal tissue
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General notes
This product was previously labelled as PCK1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab70358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). Abcam recommends using milk as the blocking agent.
Target
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Function
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. -
Tissue specificity
Major sites of expression are liver, kidney and adipocytes. -
Pathway
Carbohydrate biosynthesis; gluconeogenesis. -
Involvement in disease
Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait. -
Sequence similarities
Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. -
Post-translational
modificationsAcetylation is increased on addition of glucose and appears to regulate the protein stability. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5105 Human
- Entrez Gene: 18534 Mouse
- Entrez Gene: 362282 Rat
- Omim: 614168 Human
- SwissProt: P35558 Human
- SwissProt: Q9Z2V4 Mouse
- SwissProt: P07379 Rat
- Unigene: 1872 Human
see all -
Alternative names
- cytosolic [GTP] antibody
- GTP antibody
- PCK1 antibody
see all
Images
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Western blot - Anti-PCK1/PEPC antibody (ab70358)All lanes : Anti-PCK1/PEPC antibody (ab70358) at 1 µg
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Liver (Rat) Tissue Lysate
Lane 3 : Liver (Mouse) Tissue Lysate
Lane 4 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Additional bands at: 32 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% Milk before being incubated with ab70358 overnight at 4°C. Antibody binding was detected using an anti-rabbit VHH single domain antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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Western blot - Anti-PCK1/PEPC antibody (ab70358)This image is courtesy of an anonymous AbreviewAll lanes : Anti-PCK1/PEPC antibody (ab70358) at 1 µg/ml
All lanes : Mouse adipose tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP conjugated Donkey anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 5 hours
Datasheets and documents
References (20)
ab70358 has been referenced in 20 publications.
- Sharma AX et al. Glucagon Receptor Antagonism Improves Glucose Metabolism and Cardiac Function by Promoting AMP-Mediated Protein Kinase in Diabetic Mice. Cell Rep 22:1760-1773 (2018). WB ; Mouse . PubMed: 29444429
- Wang Z et al. Curcumin restrains hepatic glucose production by blocking cAMP/PKA signaling and reducing acetyl CoA accumulation in high-fat diet (HFD)-fed mice. Mol Cell Endocrinol 474:127-136 (2018). WB ; Mouse . PubMed: 29499209
- Zhang N et al. Elevated hepatic expression of H19 long noncoding RNA contributes to diabetic hyperglycemia. JCI Insight 3:N/A (2018). PubMed: 29769440
- Ruan JS et al. Chronic palmitic acid-induced lipotoxicity correlates with defective trafficking of ATP sensitive potassium channels in pancreatic ß cells. J Nutr Biochem 59:37-48 (2018). PubMed: 29960115
- Li A et al. Berberine Reduces Pyruvate-driven Hepatic Glucose Production by Limiting Mitochondrial Import of Pyruvate through Mitochondrial Pyruvate Carrier 1. EBioMedicine 34:243-255 (2018). PubMed: 30093307
Images
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Western blot - Anti-PCK1/PEPC antibody (ab70358)All lanes : Anti-PCK1/PEPC antibody (ab70358) at 1 µg
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Liver (Rat) Tissue Lysate
Lane 3 : Liver (Mouse) Tissue Lysate
Lane 4 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Additional bands at: 32 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% Milk before being incubated with ab70358 overnight at 4°C. Antibody binding was detected using an anti-rabbit VHH single domain antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
-
Western blot - Anti-PCK1/PEPC antibody (ab70358) This image is courtesy of an anonymous AbreviewAll lanes : Anti-PCK1/PEPC antibody (ab70358) at 1 µg/ml
All lanes : Mouse adipose tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP conjugated Donkey anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 5 hours
