Elk1 Transcription Factor Assay Kit (Colorimetric) (ab207202)
Key features and details
- Assay type: Semi-quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 3 hr 30 min
- Sample type: Nuclear Extracts
- Sensitivity: 1000 ng/well
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 5 x 96 tests 10X Antibody Binding Buffer 1 x 2.2ml 1 x 11ml 10X Wash Buffer 1 x 22ml 1 x 110ml 96-well assay plate 1 unit 5 units Anti-mouse HRP-conjugated IgG (0.25 μg/μL) 1 x 11µl 1 x 55µl Binding Buffer 1 x 10ml 1 x 50ml Developing Solution 1 x 11ml 1 x 55ml Dithiothreitol (DTT) (1 M) 1 x 100µl 1 x 500µl HeLa (TPA, 10 min) nuclear extract (2.5 μg/μL) 1 x 40µl 1 x 200µl Lysis Buffer 1 x 10ml 1 x 50ml Mutated oligonucleotide (10 pmol/μL) 1 x 100µl 1 x 500µl Phosphorylated Elk-1 antibody 1 x 11µl 1 x 55µl Plate sealer 1 unit 5 units Poly [d(l-c)] (17 µg/μL) 1 x 100µl 1 x 500µl Protease Inhibitor Cocktail 1 x 100µl 1 x 500µl Stop Solution 1 x 11ml 1 x 55ml Wild-type oligonucleotide (10 pmol/μL) 1 x 100µl 1 x 500µl -
Research areas
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Function
Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element. -
Tissue specificity
Lung and testis. -
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. -
Post-translational
modificationsSumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- ELK 1
- Elk1
- ELK1 member of ETS oncogene family
see all -
Database links
- Entrez Gene: 2002 Human
- Omim: 311040 Human
- SwissProt: P19419 Human
- Unigene: 181128 Human
Images
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Different amounts of nuclear extracts from untreated U-937 (Light gray), untreated HeLa (Dark gray) and HeLa (TPA, 10 min) (Black) cells are tested for Elk-1 activity.
Different amounts of nuclear extracts from untreated U-937 (light grey), untreated HeLa (dark grey) and HeLa treated with TPA for 10 min (black) cells were tested for Elk-1 activity. These results are provided for demonstration purposes only.