Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14304] to Germinal Center Kinase/GCK
  • Suitable for: Flow Cyt, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Germinal Center Kinase/GCK antibody [EPR14304]
    See all Germinal Center Kinase/GCK primary antibodies

  • Description

    Rabbit monoclonal [EPR14304] to Germinal Center Kinase/GCK

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Namalwa, Ramos and Human fetal brain lysates; Human hepatocellular carcinoma tissue; Ramos cells.

  • General notes

     

     

     This product was previously labelled as Germinal Center Kinase

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169)
    Western blot - Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169)
    All lanes : Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169) at 1/10000 dilution

    Lane 1 : Namalwa cell lysate
    Lane 2 : Ramos cell lysate
    Lane 3 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 92 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma labeling Germinal Center Kinase/GCK with ab184169 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169)
    Flow Cytometry - Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169)

    Flow cytometric analysis of Ramos cells (2% paraformaldehyde-fixed) labeling Germinal Center Kinase/GCK with ab184169 at 1/170 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169)
    Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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