Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LKB1 antibody [EPR19379] - BSA and Azide free (ab223146)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19379] to LKB1 - BSA and Azide free
  • Suitable for: IP, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-LKB1 antibody [EPR19379] - BSA and Azide free
    See all LKB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19379] to LKB1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T, Jurkat and K562 whole cell lysates; Human brain, human testis, fetal heart, fetal kidney and fetal testis lysates. IP: K562 and human testis lysates.

  • General notes

    Ab223146 is the carrier-free version of ab199970. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab223146 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-LKB1 antibody [EPR19379] - BSA and Azide free (ab223146)
    Western blot - Anti-LKB1 antibody [EPR19379] - BSA and Azide free (ab223146)
    All lanes : Anti-LKB1 antibody [EPR19379] (ab199970) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : STK11 knockout HEK-293T cell lysate
    Lane 3 : Hu testis cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 45, 49 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab199970).

    Lanes 1- 4: Merged signal (red and green). Green - ab199970 observed at 55 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab199970 was shown to react with LKB1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266647 (knockout cell lysate ab257712) was used. Wild-type HEK-293T and STK11 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab199970 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-LKB1 antibody [EPR19379] - BSA and Azide free (ab223146)
    Immunoprecipitation - Anti-LKB1 antibody [EPR19379] - BSA and Azide free (ab223146)

    LKB1 was immunoprecipitated from 1 mg of K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab199970 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab199970 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: K562  whole cell lysate, 10 μg (Input).

    Lane 2: ab199970 IP in K562  whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab199970 in K562 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    LKB1 isoforms: PMID 18854309.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199970).

  • Immunoprecipitation - Anti-LKB1 antibody [EPR19379] - BSA and Azide free (ab223146)
    Immunoprecipitation - Anti-LKB1 antibody [EPR19379] - BSA and Azide free (ab223146)

    LKB1 was immunoprecipitated from 1 mg of Human testis lysate with ab199970 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab199970 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Human testis lysate, 10 μg (Input).

    Lane 2: ab199970 IP in Human testis lysate .

    Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab199970 in Human testis lysate .

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    LKB1 isoforms: PMID 18854309.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199970).

  • Anti-LKB1 antibody [EPR19379] - BSA and Azide free (ab223146)
    Anti-LKB1 antibody [EPR19379] - BSA and Azide free (ab223146)

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