Human ERCC1 Matched Antibody Pair Kit (ab218181)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 125 pg/ml - 8000 pg/ml
Overview
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Product name
Human ERCC1 Matched Antibody Pair Kit
See all ERCC1 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
51.8 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human ERCC1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human ERCC1.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab210582. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human ERCC1 Capture Antibody 2 x 50µg 1 x 50µg Human ERCC1 Detector Antibody 2 x 12.5µg 1 x 12.5µg Human ERCC1 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. -
Involvement in disease
Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur. -
Sequence similarities
Belongs to the ERCC1/RAD10/SWI10 family. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- COFS 4
- COFS4
- DNA excision repair protein ERCC 1
see all -
Database links
- Entrez Gene: 2067 Human
- Omim: 126380 Human
- SwissProt: P07992 Human
- Unigene: 435981 Human
Images
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Human ERCC1 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human ERCC1 Matched Antibody Pair Kit (ab218181)To learn more about the advantages of recombinant antibodies see here.
