Human ERCC1 ELISA Kit (ab210582)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 58 pg/ml
- Range: 109 pg/ml - 7000 pg/ml
- Sample type: Cell culture extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ERCC1 ELISA Kit
See all ERCC1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Cell extract 5 6.4% Inter-assay Sample n Mean SD CV% Cell extract 3 5.4% -
Sample type
Cell culture extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
58 pg/ml -
Range
109 pg/ml - 7000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 110 109% - 111% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human ERCC1 ELISA Kit (ab210582) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ERCC1 protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ERCC1 with 58 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
ERCC1 is a DNA repair endonuclease. It plays a role in the first stage of the repair of interstrand cross-links by 5’-incision. ERCC1 is also essential for homology-directed repair of DNA double strand breaks.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human ERCC1 Capture Antibody 1 x 600µl 10X Human ERCC1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human ERCC1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. -
Involvement in disease
Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur. -
Sequence similarities
Belongs to the ERCC1/RAD10/SWI10 family. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- COFS 4
- COFS4
- DNA excision repair protein ERCC 1
see all -
Database links
- Entrez Gene: 2067 Human
- Omim: 126380 Human
- SwissProt: P07992 Human
- Unigene: 435981 Human
Images
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Other - Human ERCC1 ELISA Kit (ab210582)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human ERCC1 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native ERCC1 in human lysates based on a 500 µg/mL extract load.The concentrations of ERCC1 were measured in duplicate and interpolated from the ERCC1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ERCC1 concentration was determined to be 1,146 pg/mL in HepG2 extracts and 490 pg/mL in HUVEC extracts.
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Sandwich ELISA - Human ERCC1 ELISA Kit (ab210582)To learn more about the advantages of recombinant antibodies see here.
