Anti-NDUFA5 antibody (ab241492)
Key features and details
- Rabbit polyclonal to NDUFA5
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NDUFA5 antibody
See all NDUFA5 primary antibodies -
Description
Rabbit polyclonal to NDUFA5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human NDUFA5 aa 66-116. The exact sequence is proprietary.
Database link: Q16718 -
Positive control
- WB: HeLa, HEK-293T, Jurkat and TCMK-1 whole cell lysate. IP: NDUFA5 IP in HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to NDUFA5 immobilized on solid support -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-NDUFA5 antibody (ab241492)
NDUFA5 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241492 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab241492 at 0.4 µg/ml.
Lane 1: ab241492 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with an exposure time of 75 seconds.
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Western blot - Anti-NDUFA5 antibody (ab241492)All lanes : Anti-NDUFA5 antibody (ab241492) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 30 secondsPrepared using NETN lysis buffer
