Recombinant Human Alpha-synuclein protein monomer (Type 2) (ab218816)
Key features and details
- Expression system: Escherichia coli
- Purity: = 92% SDS-PAGE
- Endotoxin level:
- Suitable for: SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: PBS
0.2µm filtered solution
Images
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SDS-PAGE analysis of ab218816 at 2.5 µg (Lane 3) or 5 µg (Lane 4). BSA at 2.5 µg (Lane 1) and 5 µg (Lane 2) is shown for comparison.
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ThT emission curves show increased fluorescence (correlated to alpha-synucleinprotein aggregation) over time when 10 µM of active alpha-synuclein aggregate (ab218819) is combined with 100 µM of active alpha-synuclein monomer (ab218818) (light blue), as compared to when 100 µM of active alpha-synuclein monomer is combined with 10 µM of control alpha-synuclein aggregate (purple line), or 100 µM of control alpha-synuclein monomer (ab218816) is combined with 10 µM of control alpha-synuclein aggregate (ab218817) (dark blue). ThT ex = 450 nm, em = 485 nm. View protocol.