Anti-Alpha-synuclein (phospho Y133) antibody (ab51104)
Key features and details
- Rabbit polyclonal to Alpha-synuclein (phospho Y133)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Alpha-synuclein (phospho Y133) antibody
See all Alpha-synuclein primary antibodies -
Description
Rabbit polyclonal to Alpha-synuclein (phospho Y133) -
Host species
Rabbit -
Specificity
ab51104 detects endogenous levels of alpha Synuclein only when phosphorylated at tyrosine 133. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Alpha-synuclein. A synthetic phospho-peptide derived from human alpha Synuclein around the phosphorylation site of tyrosine 133 (E-G-YP-Q-D).
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Positive control
- Extracts from 293 cells treated with Etoposide (25µM, 60min)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab51104 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Alpha-synuclein (phospho Y133) antibody (ab51104)All lanes : Anti-Alpha-synuclein (phospho Y133) antibody (ab51104) at 1/500 dilution
Lane 1 : 293 cell extract treated with
Etoposide (25µM, 60min)
Lane 2 : 293 cell extract treated with
Etoposide (25µM, 60min) and immunizing peptide
Predicted band size: 14 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho Y133) antibody (ab51104)Ab51104 staining Human dentate nucleus. Staining is localised to the cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
