Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [42/a-Synuclein] to Alpha-synuclein - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free
    See all Alpha-synuclein primary antibodies

  • Description

    Mouse monoclonal [42/a-Synuclein] to Alpha-synuclein - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC or IHC-Fr

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • WB: Mouse cerebellum, Human cerebellum, Rat hippocampus. IHC-P: Rat cerebrum, Mouse cerebrum, Human cerebrum.

  • General notes

    ab280382 is the carrier-free version of ab280377. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280382 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)
    Western blot - Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)
    All lanes : Anti-Alpha-synuclein antibody [42/a-Synuclein] (ab280377) at 1/1000 dilution

    Lane 1 : Mouse cerebellum lysate at 20 µg
    Lane 2 : Mouse kidney lysate at 20 µg
    Lane 3 : Human cerebellum lysate at 20 µg
    Lane 4 : Human kidney lysate at 20 µg
    Lane 5 : Rat hippocampus lysate at 20 µg
    Lane 6 : Rat kidney lysate at 10 µg

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/1000 dilution

    Predicted band size: 14 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab280377, the same antibody clone in a different buffer formulation.

    Blocking and Diluting buffer and concentration: 5% NFDM/TBST

    Negative control: mouse kidney, human kidney, rat kidney (PMID:14997013)

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:14997013, 30553024).

    Exposure time 15 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)

    This data was developed using ab280377 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Alpha-synuclein with abab280377 at 1/5000 (0.221 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining in human cerebrum.The section was incubated with ab280377 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)

    This data was developed using ab280377 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Alpha-synuclein with abab280377 at 1/5000 (0.221 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining in mouse cerebrum.The section was incubated with ab280377 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)

    This data was developed using ab280377 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Alpha-synuclein with abab280377 at 1/5000 (0.221 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining in rat cerebrum.The section was incubated with ab280377 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)
    Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)

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