Anti-PGC1 alpha + beta antibody (ab72230)
Key features and details
- Rabbit polyclonal to PGC1 alpha + beta
- Suitable for: ELISA, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PGC1 alpha + beta antibody
See all PGC1 alpha + beta primary antibodies -
Description
Rabbit polyclonal to PGC1 alpha + beta -
Host species
Rabbit -
Specificity
The immunogen design for ab72230 is shown to cross-react with both PGC1 alpha (ID: 124782 and Swissprot Q9UBK2) and PGC1 beta (ID: 240776 and SwissProt Q86YN6). -
Tested applications
Suitable for: ELISA, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Dog, Pig, Rhesus monkey -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human PGC1 alpha.
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Positive control
- This antibody gave a positive signal in the following Tissue Lysates: Human Skeletal Muscle Mouse Testis Mouse Colon Mouse Skeletal Muscle Rat Ovary Rat Thymus
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General notes
PGC1 alpha has a predicted MW of 92 kDa and PGC1 beta has a predicted MW of 113 kDa.
This product may be covered by one or more of the following U.S. Patents.
6,166,192
6,426,411
6,525,178
6,872,391
6,908,987
7,250,283
7,091,006
7,354,738
8,105,774
8,445,233Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Images
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Immunofluorescence analysis of 4% paraformaldehyde fixed HeLa cells (Human epithelial cells from cervix adenocarcinoma) labeling PGC1 alpha+beta with ab72230 at a 1/100 dilution showing nuclear staining. Permeabilisation using 0.1% tritonX-100.
Secondary ab: Anti-Rabbit Alexa Fluor® 488 (ab150077) at 1/500 dilution. Counter stain is labeling tubulin (red) with ab7291 at 1/1000 dilution with secondary antibody Anti-Mouse AlexaFluor® 594 (ab150120) at 1/500 dilution. DAPI stains the nucleus in blue. -ve control 1 is ab72230 at 1/100 dilution, Anti-Mouse AlexaFluor® 594 (ab150120) at 1/500 dilution. -ve control 2 is ab7291 at 1/1000 dilution, Anti-Rabbit Alexa Fluor® 488 (ab150077) at 1/500 dilution.
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All lanes : Anti-PGC1 alpha + beta antibody (ab72230) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate
Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 3 : Ovary (Rat) Tissue Lysate
Lane 4 : Colon (Mouse) Tissue Lysate
Lane 5 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 6 : Thymus (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 91 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa, 45 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
We have occasionally observed bands in the region of 90 kDa in batches of this antibody, which corresponds to the molecular weight based on primary sequence. -
All lanes : Anti-PGC1 alpha + beta antibody (ab72230) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Colon (Mouse) Tissue Lysate
Lane 3 : Thymus (Rat) Tissue Lysate
Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330) with Human PGC1 alpha peptide (ab73600) at 1 µg/ml
Lane 5 : Colon (Mouse) Tissue Lysate with Human PGC1 alpha peptide (ab73600) at 1 µg/ml
Lane 6 : Thymus (Rat) Tissue Lysate with Human PGC1 alpha peptide (ab73600) at 1 µg/ml
Lane 7 : Human skeletal muscle tissue lysate - total protein (ab29330) with PGC1 beta peptide at 1 µg/ml
Lane 8 : Colon (Mouse) Tissue Lysate with PGC1 beta peptide at 1 µg/ml
Lane 9 : Thymus (Rat) Tissue Lysate with PGC1 beta peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 91 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa, 73 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Western Blot showing equal blocking with the PGC1 alpha and PGC1 beta peptides. -
ab72230 was tested using an Indirect ELISA approach. The wells were coated with peptide (1µg/ml at 100µl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025µg/ml (100µl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.
Ab72230 gave a positive result in ELISA against PGC1 alpha peptide and PGC1 beta peptide. This indicates that it recognises both forms.
Orange line: ELISA against immunising peptide ab73600
Yellow line: ELISA against ab88232