Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free
See all PGC1 alpha + beta primary antibodies
Description

Rabbit monoclonal [EPR18289] to PGC1 alpha + beta - BSA and Azide free
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Recombinant fragment

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
ab250943 is the carrier-free version of ab188102 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab250943 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

This product may be covered by one or more of the following U.S. Patents.

6,166,192
6,426,411
6,525,178
6,872,391
6,908,987
7,250,283
7,091,006
7,354,738
8,105,774
8,445,233
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Recombinant fragment

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Clonality

Monoclonal
Clone number

EPR18289
Isotype

IgG
Research areas

Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)

Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/100000 dilution + Recombinant fragment of Human PGC1 alpha protein at 0.01 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 91, 113 kDa
Observed band size: 38 kDa
why is the actual band size different from the predicted?

Exposure time: 1 minute

This data was developed using ab188102, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Recombinant fragment of human PGC1 alpha protein contains aa459-798 with His-Tag^®.
Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)

Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/100000 dilution + Recombinant fragment of Human PGC1 beta protein at 0.01 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 91, 113 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?

Exposure time: 15 seconds

This data was developed using ab188102, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Recombinant fragment of human PGC1 beta protein contains aa751-1023 with His-Tag^Â®.
Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)

All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/1000 dilution

Lane 1 : Human skeletal muscle tissue lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 3 : Mouse colon tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 91, 113 kDa
Observed band size: 90, 113 kDa why is the actual band size different from the predicted?

This data was developed using ab188102, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

The composite image shows duplicate western blots with different exposure times. A 90 KDa band (arrow), likely to be PGC1 alpha, is detected at a longer exposure time (1 min). It has been reported that the skeletal muscle, heart and kidney have a higher expression level of PGC1 alpha than other tissues (Kressler et al. 2002, J. Biol. Chem. 277, 13918-13925).
Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)

All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/10000 dilution

Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lane 3 : Mouse thymus tissue lysate
Lane 4 : Rat thymus tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 91, 113 kDa
Observed band size: 113 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

This data was developed using ab188102, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)

All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/10000 dilution

Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat heart tissue lysate
Lane 5 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 91, 113 kDa
Observed band size: 113 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

This data was developed using ab188102, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)

All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/1000 dilution

Lane 1 : Mouse kidney lysate
Lane 2 : Rat kidney lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 91, 113 kDa
Observed band size: 113 kDa why is the actual band size different from the predicted?

Exposure time: 5 seconds

This data was developed using ab188102, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

A high expression level of PGC1 alpha in the kidney has been reported (Kressler et al. 2002, J. Biol. Chem. 277, 13918–13925). The lower band in lane 2 is likely to be the alpha form.
Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)

All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/1000 dilution

Lane 1 : Human skeletal muscle lysate
Lane 2 : Rat kidney lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1000 µg

Predicted band size: 91, 113 kDa
Observed band size: 113 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

This data was developed using ab188102, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

A high expression level of PGC1 alpha in the kidney has been reported (Kressler et al. 2002, J. Biol. Chem. 277, 13918-13925). The lower band in lane 2 is likely to be the alpha form.
Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)