Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18289] to PGC1 alpha + beta - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free
    See all PGC1 alpha + beta primary antibodies

  • Description

    Rabbit monoclonal [EPR18289] to PGC1 alpha + beta - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250943 is the carrier-free version of ab188102 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250943 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product may be covered by one or more of the following U.S. Patents.

    6,166,192
    6,426,411
    6,525,178
    6,872,391
    6,908,987
    7,250,283
    7,091,006
    7,354,738
    8,105,774
    8,445,233

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)
    Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)
    Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/100000 dilution + Recombinant fragment of Human PGC1 alpha protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 91, 113 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This data was developed using ab188102, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Recombinant fragment of human PGC1 alpha protein contains aa459-798 with His-Tag®.

  • Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)
    Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)
    Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/100000 dilution + Recombinant fragment of Human PGC1 beta protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 91, 113 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This data was developed using ab188102, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Recombinant fragment of human PGC1 beta protein contains aa751-1023 with His-Tag®.

  • Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)
    Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)
    All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/1000 dilution

    Lane 1 : Human skeletal muscle tissue lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Mouse colon tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 91, 113 kDa
    Observed band size: 90, 113 kDa why is the actual band size different from the predicted?



    This data was developed using ab188102, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The composite image shows duplicate western blots with different exposure times. A 90 KDa band (arrow), likely to be PGC1 alpha, is detected at a longer exposure time (1 min). It has been reported that the skeletal muscle, heart and kidney have a higher expression level of PGC1 alpha than other tissues (Kressler et al. 2002, J. Biol. Chem. 277, 13918-13925).

  • Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)
    Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)
    All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/10000 dilution

    Lane 1 : Human fetal heart tissue lysate
    Lane 2 : Human fetal kidney tissue lysate
    Lane 3 : Mouse thymus tissue lysate
    Lane 4 : Rat thymus tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 91, 113 kDa
    Observed band size: 113 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab188102, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)
    Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)
    All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/10000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : Rat heart tissue lysate
    Lane 5 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 91, 113 kDa
    Observed band size: 113 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This data was developed using ab188102, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)
    Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)
    All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/1000 dilution

    Lane 1 : Mouse kidney lysate
    Lane 2 : Rat kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 91, 113 kDa
    Observed band size: 113 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    This data was developed using ab188102, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    A high expression level of PGC1 alpha in the kidney has been reported (Kressler et al. 2002, J. Biol. Chem. 277, 13918–13925). The lower band in lane 2 is likely to be the alpha form.

  • Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)
    Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)
    All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/1000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Rat kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1000 µg

    Predicted band size: 91, 113 kDa
    Observed band size: 113 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This data was developed using ab188102, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    A high expression level of PGC1 alpha in the kidney has been reported (Kressler et al. 2002, J. Biol. Chem. 277, 13918-13925). The lower band in lane 2 is likely to be the alpha form.

  • Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)
    Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-PGC1 alpha + beta antibody [EPR18289] - BSA and Azide free (ab250943)

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.