Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cathepsin B antibody [EPR4323] - BSA and Azide free (ab230401)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4323] to Cathepsin B - BSA and Azide free
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Cathepsin B antibody [EPR4323] - BSA and Azide free
    See all Cathepsin B primary antibodies

  • Description

    Rabbit monoclonal [EPR4323] to Cathepsin B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Cathepsin B aa 1-200. The exact sequence is proprietary.

  • Positive control

    • Human kidney and papillary carcinoma tissues; HeLa cells; HT1080, A375, SW480 and A549 cell line lysates.

  • General notes

    Ab230401 is the carrier-free version of ab125067. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab230401 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] - BSA and Azide free (ab230401)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] - BSA and Azide free (ab230401)

    ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human kidney tissue by immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab125067).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] - BSA and Azide free (ab230401)
    Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] - BSA and Azide free (ab230401)
    ab125067, at a 1/50 dilution, staining Cathepsin B (red) in HeLa cells by immunofluorescence. DNA is marked in blue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab125067).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] - BSA and Azide free (ab230401)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] - BSA and Azide free (ab230401)

    This IHC data was generated using the same anti-Cathepsin B antibody clone, EPR4323, in a different buffer formulation (cat# ab125067).

    ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human papillary carcinoma tissue by immunohistochemistry.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] - BSA and Azide free (ab230401)
    Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] - BSA and Azide free (ab230401)

    This ICC data was generated using the same anti-Cathepsin B antibody clone, EPR4323, in a different buffer formulation (cat# ab125067).

    ab125067, at a 1/50 dilution, staining Cathepsin B (red) in HeLa cells by immunofluorescence. DNA is marked in blue.

  • Anti-Cathepsin B antibody [EPR4323] - BSA and Azide free (ab230401)
    Anti-Cathepsin B antibody [EPR4323] - BSA and Azide free (ab230401)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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