Anti-Cathepsin B antibody [CA10] (ab58802)
![Anti-Cathepsin B antibody [CA10] (ab58802)](https://www.abcam.com/ps/products/58/ab58802/Images/ab58802-72704-anti-cathepsin-b-antibody-ca10-western-blot.jpg "Anti-Cathepsin B antibody [CA10] (ab58802)")
Key features and details
- Mouse monoclonal [CA10] to Cathepsin B
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Cathepsin B antibody [CA10]
See all Cathepsin B primary antibodies -
Description
Mouse monoclonal [CA10] to Cathepsin B -
Host species
Mouse -
Specificity
This antibody recognizes an epitope on the heavy chain of mature Cathepsin B and therefore detects both mature (~31 kDa) and pro (~43 kDa)Cathepsin B. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Recombinant fragment
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Immunogen
Cathepsin B purified from human liver
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Epitope
Within the heavy chain of mature cathepsin B -
Positive control
- Flow cyt: HepG2 cells
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General notes
This product has switched from ascites to TCS on 26th October 2020.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Constituents: 0.158% Ammonium bicarbonate, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
CA10 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-Cathepsin B antibody [CA10] (ab58802)Anti-Cathepsin B antibody [CA10] (ab58802) at 0.25 µg/ml +
Native human Cathepsin B protein (ab90387) at 0.01 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Exposure time: 10 seconds -
![Flow Cytometry - Anti-Cathepsin B antibody [CA10] (ab58802)](https://www.abcam.com/ps/products/58/ab58802/Images/ab58802-72703-anti-cathepsin-b-antibody-ca10-flow-cytometry.jpg)
Flow Cytometry - Anti-Cathepsin B antibody [CA10] (ab58802)Overlay histogram showing HepG2 cells stained with ab58802 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58802, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
