Antibodies, Proteins, Kits and Reagents for Life Science

324 988 ₸ Product size

100 µl 324 988 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4330] to CD41
Suitable for: ICC/IF, WB, IHC-P
Reacts with: Mouse, Human

Product name

Anti-CD41 antibody [EPR4330]
See all CD41 primary antibodies
Description

Rabbit monoclonal [EPR4330] to CD41
Host species

Rabbit
Specificity

Mouse reactivity is only guaranteed for IHC-P.

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human CD41 aa 50-150 (extracellular). The exact sequence is proprietary. Synthetic peptide corresponding to residues in the extracellular domain in Human CD41.
Database link: P08514
Positive control
- Human platelet, fetal liver and placenta lysates; Human spleen tissue
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% Tris glycine, 0.88% Sodium chloride, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4330
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131)

Ab134131 staining CD41 in paraffin embedded Human bone marrow tissue sections by Immunohistochemistry. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/2000 dilution (0.30 μg/ml). A ready to use Goat Anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining in megakaryocytes of human bone marrow (PMID: 27128503; PMID: 23667055).
Western blot - Anti-CD41 antibody [EPR4330] (ab134131)

All lanes : Anti-CD41 antibody [EPR4330] (ab134131) at 0.6 µg/ml

Lane 1 : HEL (human Erythroleukemia erythroblast) whole cell lysate
Lane 2 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 113 kDa

Exposure time: 3 minutes

Blocking and diluting buffer: 5% NFDM/TBST.

Negative control: K-562 (PMID 2458779)
Immunocytochemistry/ Immunofluorescence - Anti-CD41 antibody [EPR4330] (ab134131)

Ab134131 staining CD41 in HEL (human Erythroleukemia erythroblast) cells by ICC/IF (Immunocytochemistry/Immunofluorescence. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1/100 dilution (6 μg/ml). An AlexaFluor^® 488 Goat anti-Rabbit (ab150077) was used as the secondary antibody at 1/1000 dilution (2 μg/ml). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) , ab195889 was used a counterstain antibody at 1/200 dilution (2.5 μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing membranous staining in HEL cell line.

Negative control: K562( PMID 2458779)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131)

Ab134131 staining CD41 paraffin embedded Mouse bone marrow tissue sections by Immunohistochemistry. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/500 dilution (1.21 μg/ml). A ready to use Goat Anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining in megakaryocytes of mouse bone marrow (PMID: 27128503).
Western blot - Anti-CD41 antibody [EPR4330] (ab134131)

All lanes : Anti-CD41 antibody [EPR4330] (ab134131) at 1/1000 dilution

Lane 1 : Human platelet lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human placenta lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

Predicted band size: 113 kDa
Anti-CD41 antibody [EPR4330] (ab134131)
Anti-CD41 antibody [EPR4330] (ab134131)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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