Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD41 antibody [EPR4330] (ab134131)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4330] to CD41
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CD41 antibody [EPR4330]
    See all CD41 primary antibodies

  • Description

    Rabbit monoclonal [EPR4330] to CD41

  • Host species

    Rabbit

  • Specificity

    Mouse reactivity is only guaranteed for IHC-P. 

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CD41 aa 50-150 (extracellular). The exact sequence is proprietary. Synthetic peptide corresponding to residues in the extracellular domain in Human CD41.
    Database link: P08514

  • Positive control

    • Human platelet, fetal liver and placenta lysates; Human spleen tissue

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131)

    Ab134131 staining CD41 in paraffin embedded Human bone marrow tissue sections by Immunohistochemistry. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/2000 dilution (0.30 μg/ml).  A ready to use Goat Anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining in megakaryocytes of human bone marrow (PMID: 27128503; PMID: 23667055).

  • Western blot - Anti-CD41 antibody [EPR4330] (ab134131)
    Western blot - Anti-CD41 antibody [EPR4330] (ab134131)
    All lanes : Anti-CD41 antibody [EPR4330] (ab134131) at 0.6 µg/ml

    Lane 1 : HEL (human Erythroleukemia erythroblast) whole cell lysate
    Lane 2 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 113 kDa


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST. 

    Negative control: K-562 (PMID 2458779)

  • Immunocytochemistry/ Immunofluorescence - Anti-CD41 antibody [EPR4330] (ab134131)
    Immunocytochemistry/ Immunofluorescence - Anti-CD41 antibody [EPR4330] (ab134131)

    Ab134131 staining CD41 in HEL (human Erythroleukemia erythroblast) cells by ICC/IF (Immunocytochemistry/Immunofluorescence. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1/100 dilution (6 μg/ml). An AlexaFluor® 488 Goat anti-Rabbit (ab150077) was used as the secondary antibody at 1/1000 dilution (2 μg/ml). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) , ab195889 was used a counterstain antibody at 1/200 dilution (2.5 μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing membranous staining in HEL cell line.

    Negative control: K562( PMID 2458779)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131)

    Ab134131 staining CD41 paraffin embedded Mouse bone marrow tissue sections by Immunohistochemistry. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/500 dilution (1.21 μg/ml). A ready to use Goat Anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining in megakaryocytes of mouse bone marrow (PMID: 27128503).

  • Western blot - Anti-CD41 antibody [EPR4330] (ab134131)
    Western blot - Anti-CD41 antibody [EPR4330] (ab134131)
    All lanes : Anti-CD41 antibody [EPR4330] (ab134131) at 1/1000 dilution

    Lane 1 : Human platelet lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : Human placenta lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

    Predicted band size: 113 kDa

  • Anti-CD41 antibody [EPR4330] (ab134131)
    Anti-CD41 antibody [EPR4330] (ab134131)
  • Anti-CD41 antibody [EPR4330] (ab134131)
    Anti-CD41 antibody [EPR4330] (ab134131)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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