Anti-PDSS2 antibody [OTI1D12] (ab119768)
![Anti-PDSS2 antibody [OTI1D12] (ab119768)](https://www.abcam.com/ps/products/119/ab119768/Images/ab119768-132998-anti-pdss2-antibody-oti1d12-immunohistochemistry.jpg "Anti-PDSS2 antibody [OTI1D12] (ab119768)")
Key features and details
- Mouse monoclonal [OTI1D12] to PDSS2
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Dog, Human, African green monkey
- Isotype: IgG1
Overview
-
Product name
Anti-PDSS2 antibody [OTI1D12]
See all PDSS2 primary antibodies -
Description
Mouse monoclonal [OTI1D12] to PDSS2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
-
Immunogen
Recombinant full length protein corresponding to Human PDSS2 aa 1-400. Produced in HEK-293T cells (NP_065114).
Database link: Q86YH6 -
Positive control
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY PDSS2 cDNA; HepG2 and COS7 cell extracts. IHC-P: Human kidney, lung carcinoma, endometrium adenocarcinoma and lymphoma tissues. Flow Cyt: HEK-293T cells transfected with PDSS2 overexpress plasmid.
-
General notes
Clone OTI1D12 (formerly 1D12).
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant -
Clonality
Monoclonal -
Clone number
OTI1D12 -
Isotype
IgG1 -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDSS2 antibody [OTI1D12] (ab119768)
Paraffin-embedded human kidney tissue stained for PDSS2 using ab119768 at 1/150 dilution in immunohistochemical analysis.
-
![Western blot - Anti-PDSS2 antibody [OTI1D12] (ab119768)](https://www.abcam.com/ps/products/119/ab119768/Images/ab119768-133000-anti-pdss2-antibody-oti1d12-western-blot.jpg)
Western blot - Anti-PDSS2 antibody [OTI1D12] (ab119768)All lanes : Anti-PDSS2 antibody [OTI1D12] (ab119768) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY PDSS2 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 44 kDa
-
![Western blot - Anti-PDSS2 antibody [OTI1D12] (ab119768)](https://www.abcam.com/ps/products/119/ab119768/Images/ab119768-132999-anti-pdss2-antibody-oti1d12-western-blot.jpg)
Western blot - Anti-PDSS2 antibody [OTI1D12] (ab119768)All lanes : Anti-PDSS2 antibody [OTI1D12] (ab119768) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : SVT2 cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (canine kidney cell line) cell extract
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 44 kDa
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDSS2 antibody [OTI1D12] (ab119768)](https://www.abcam.com/ps/products/119/ab119768/Images/ab119768-132997-anti-pdss2-antibody-oti1d12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDSS2 antibody [OTI1D12] (ab119768)Paraffin-embedded human lung carcinoma tissue stained for PDSS2 using ab119768 at 1/150 dilution in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDSS2 antibody [OTI1D12] (ab119768)](https://www.abcam.com/ps/products/119/ab119768/Images/ab119768-132996-anti-pdss2-antibody-oti1d12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDSS2 antibody [OTI1D12] (ab119768)Paraffin-embedded human endometrium adenocarcinoma tissue stained for PDSS2 using ab119768 at 1/150 dilution in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDSS2 antibody [OTI1D12] (ab119768)](https://www.abcam.com/ps/products/119/ab119768/Images/ab119768-132995-anti-pdss2-antibody-oti1d12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDSS2 antibody [OTI1D12] (ab119768)Paraffin-embedded human lymphoma tissue stained for PDSS2 using ab119768 at 1/150 dilution in immunohistochemical analysis.
-
![Flow Cytometry - Anti-PDSS2 antibody [OTI1D12] (ab119768)](https://www.abcam.com/ps/products/119/ab119768/Images/ab119768-132994-anti-pdss2-antibody-oti1d12-flow-cytometry.jpg)
Flow Cytometry - Anti-PDSS2 antibody [OTI1D12] (ab119768)Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either PDSS2 overexpress plasmid (Red) or empty vector control plasmid (Blue) labeling pdss2 with ab119768 at 1/100 dilution.
