Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDK4 antibody [EPR19727-245] (ab214938)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19727-245] to PDK4
  • Suitable for: IP, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Recombinant fragment

Overview

  • Product name

    Anti-PDK4 antibody [EPR19727-245]
    See all PDK4 primary antibodies

  • Description

    Rabbit monoclonal [EPR19727-245] to PDK4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    IP
    Mouse
    WB
    Mouse
    Rat
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse PDK4 recombinant protein; mouse skeletal muscle and heart lysates; rat muscle and heart lysates; C2C12 and C6 whole cell lysate; heart from nonfasting mouse and mouse fasting for 72 hours; gastrocnemius muscle from nonfasting mouse and mouse fasting for 72 hours. Flow Cyt: C2C12 cells. IP: Mouse heart lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938)
    Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938)
    All lanes : Anti-PDK4 antibody [EPR19727-245] (ab214938) at 1/10000 dilution

    Lane 1 : Mouse PDK4 recombinant protein
    Lane 2 : Mouse PDK1 recombinant protein
    Lane 3 : Mouse PDK2 recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Mouse PDK4, PDK1 and PDK2 recombinant proteins contain aa8-412, aa1-434 and aa1-407 respectively with a His-Tag®.

  • Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938)
    Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938)
    All lanes : Anti-PDK4 antibody [EPR19727-245] (ab214938) at 1/1000 dilution

    Lane 1 : Mouse skeletal muscle lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Rat muscle lysate
    Lane 4 : Rat heart lysate
    Lane 5 : C2C12 (Mouse myoblast cell line) whole cell lysate
    Lane 6 : C6 (Rat glial tumor cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 47 kDa
    Additional bands at: 47 kDa. We are unsure as to the identity of these extra bands.



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 5 seconds; Lane 2-4: 3 minutes; Lane 5: 1 minutes; Lane 6: 10 seconds.

  • Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938)
    Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938)
    Lanes 1-2 : Anti-PDK4 antibody [EPR19727-245] (ab214938) at 1/10000 dilution
    Lanes 3-4 : Anti-PDK4 antibody [EPR19727-245] (ab214938) at 1/1000 dilution

    Lane 1 : Heart from nonfasting mouse
    Lane 2 : Heart from mouse fasting for 72 hours
    Lane 3 : Gastrocnemius muscle from nonfasting mouse
    Lane 4 : Gastrocnemius muscle from mouse fasting for 72 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression level of PDK4 is increased under the fasting state (PMID: 11723055 and PMID: 16606348).

  • Flow Cytometry - Anti-PDK4 antibody [EPR19727-245] (ab214938)
    Flow Cytometry - Anti-PDK4 antibody [EPR19727-245] (ab214938)

    Flow cytometric analysis of 4% paraformaldehyde-fixed C2C12 (Mouse myoblast cell line) cells labeling PDK4 with ab214938 at 1/60 dilution (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-PDK4 antibody [EPR19727-245] (ab214938)
    Immunoprecipitation - Anti-PDK4 antibody [EPR19727-245] (ab214938)

    PDK4 was immunoprecipitated from 0.35 mg of mouse heart lysate with ab214938  at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab214938 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: Mouse heart lysate 10µg (Input).

    Lane 2: ab214938 IP in mouse heart lysate.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab214938 in mouse heart lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 0.5 second.

  • Anti-PDK4 antibody [EPR19727-245] (ab214938)
    Anti-PDK4 antibody [EPR19727-245] (ab214938)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-PDK4 antibody [EPR19727-245] (ab214938)

© 2021 Astana Biomed Group LLP. All rights reserved.