Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PDK4 antibody [EPR19727-245] (ab214938)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19727-245] to PDK4
Suitable for: IP, Flow Cyt, WB
Reacts with: Mouse, Rat, Recombinant fragment

Product name

Anti-PDK4 antibody [EPR19727-245]
See all PDK4 primary antibodies
Description

Rabbit monoclonal [EPR19727-245] to PDK4
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
IP	Mouse
WB	Mouse Rat Recombinant fragment

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse PDK4 recombinant protein; mouse skeletal muscle and heart lysates; rat muscle and heart lysates; C2C12 and C6 whole cell lysate; heart from nonfasting mouse and mouse fasting for 72 hours; gastrocnemius muscle from nonfasting mouse and mouse fasting for 72 hours. Flow Cyt: C2C12 cells. IP: Mouse heart lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19727-245
Isotype

IgG
Research areas

Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938)

All lanes : Anti-PDK4 antibody [EPR19727-245] (ab214938) at 1/10000 dilution

Lane 1 : Mouse PDK4 recombinant protein
Lane 2 : Mouse PDK1 recombinant protein
Lane 3 : Mouse PDK2 recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 47 kDa
Observed band size: 47 kDa

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Mouse PDK4, PDK1 and PDK2 recombinant proteins contain aa8-412, aa1-434 and aa1-407 respectively with a His-Tag®.
Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938)

All lanes : Anti-PDK4 antibody [EPR19727-245] (ab214938) at 1/1000 dilution

Lane 1 : Mouse skeletal muscle lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat muscle lysate
Lane 4 : Rat heart lysate
Lane 5 : C2C12 (Mouse myoblast cell line) whole cell lysate
Lane 6 : C6 (Rat glial tumor cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 47 kDa
Additional bands at: 47 kDa. We are unsure as to the identity of these extra bands.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 5 seconds; Lane 2-4: 3 minutes; Lane 5: 1 minutes; Lane 6: 10 seconds.
Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938)

Lanes 1-2 : Anti-PDK4 antibody [EPR19727-245] (ab214938) at 1/10000 dilution
Lanes 3-4 : Anti-PDK4 antibody [EPR19727-245] (ab214938) at 1/1000 dilution

Lane 1 : Heart from nonfasting mouse
Lane 2 : Heart from mouse fasting for 72 hours
Lane 3 : Gastrocnemius muscle from nonfasting mouse
Lane 4 : Gastrocnemius muscle from mouse fasting for 72 hours

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 47 kDa
Observed band size: 47 kDa

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression level of PDK4 is increased under the fasting state (PMID: 11723055 and PMID: 16606348).
Flow Cytometry - Anti-PDK4 antibody [EPR19727-245] (ab214938)

Flow cytometric analysis of 4% paraformaldehyde-fixed C2C12 (Mouse myoblast cell line) cells labeling PDK4 with ab214938 at 1/60 dilution (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-PDK4 antibody [EPR19727-245] (ab214938)

PDK4 was immunoprecipitated from 0.35 mg of mouse heart lysate with ab214938 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab214938 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

Lane 1: Mouse heart lysate 10µg (Input).

Lane 2: ab214938 IP in mouse heart lysate.

Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab214938 in mouse heart lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 0.5 second.
Anti-PDK4 antibody [EPR19727-245] (ab214938)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com