Anti-beta IV Tubulin antibody (ab222822)
Key features and details
- Rabbit polyclonal to beta IV Tubulin
- Suitable for: ICC/IF, IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-beta IV Tubulin antibody
See all beta IV Tubulin primary antibodies -
Description
Rabbit polyclonal to beta IV Tubulin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Cynomolgus monkey
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Immunogen
Recombinant full length protein corresponding to Human beta IV Tubulin aa 1 to the C-terminus.
Database link: P04350 -
Positive control
- WB: 293T and HeLa whole cell lysates. IP: HeLa cell whole lysate. ICC/IF: MCF7 cells. IHC-P: Human ovarian cancer tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-beta IV Tubulin antibody (ab222822)All lanes : Anti-beta IV Tubulin antibody (ab222822) at 1/500 dilution
Lane 1 : 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Predicted band size: 50 kDa
Observed band size: 50,58 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody (ab222822)MCF7 (human breast adenocarcinoma cell line) cells stained for beta IV Tubulin (green) using ab222822 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody (ab222822)Paraffin-embedded human ovarian cancer tissue stained for beta IV Tubulin using ab222822 at 1/100 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-beta IV Tubulin antibody (ab222822)beta IV Tubulin was immunoprecipitated from 0.5 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab222822 at 1/200 dilution. Western blot was performed from the immunoprecipitate using ab222822 at 1/500 dilution.
Lane 1: Rabbit monoclonal IgG (1 µg) instead of ab222822 in Hela whole cell lysate.Lane 2: ab222822 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate (10 μg).
