Anti-Tubulin antibody - Loading Control (ab59680)
Key features and details
- Rabbit polyclonal to Tubulin - Loading Control
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Tubulin antibody - Loading Control
See all Tubulin primary antibodies -
Description
Rabbit polyclonal to Tubulin - Loading Control -
Host species
Rabbit -
Specificity
ab59680 recognises alpha/beta-tubulin. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to a sequence of human Tubulin.
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Positive control
- HeLa cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol, 0.5% BSA -
Concentration information loading... -
Purification notes
ab59680 is affinity purified. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Tubulin antibody - Loading Control (ab59680)Anti-Tubulin antibody - Loading Control (ab59680) at 4 µg/ml + HeLa cell lysate
Predicted band size: 55 kDa
Observed band size: 55 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Tubulin antibody - Loading Control (ab59680)ICC/IF image of ab59680 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59680, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
