Anti-DYNC1I2 antibody (ab204422)
Key features and details
- Rabbit polyclonal to DYNC1I2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DYNC1I2 antibody
See all DYNC1I2 primary antibodies -
Description
Rabbit polyclonal to DYNC1I2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan -
Immunogen
Recombinant fragment corresponding to Human DYNC1I2 aa 100-200.
Database link: Q13409 -
Positive control
- WB: U-2 OS cell lysate. IHC-P: Human small intestine, testis, and kidney tissues. ICC/IF: HEK293 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small intestine tissue labelling DYNC1I2 with ab204422 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of HEK 293 cells (PFA-fixed/Triton X-100 permeabilized) labeling DYNC1I2 with ab204422 at 4 µg/ml (green).
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All lanes : Anti-DYNC1I2 antibody (ab204422) at 0.4 µg/ml
Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) transfected with control siRNA, cell lysate
Lane 2 : U-2 OS cells transfected with target specific siRNA probe #1
Lane 3 : U-2 OS cells transfected with target specific siRNA probe #2
Predicted band size: 71 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling DYNC1I2 with ab204422 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling DYNC1I2 with ab204422 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling DYNC1I2 with ab204422 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.