Recombinant human FGFR1 protein (Active) (ab168696)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: ELISA, Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 400 ug/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage
Images
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ELISA - Recombinant human FGFR1 protein (Active) (ab168696)
Immobilized ab155734 at 5 µg/mL (100 µL/well) can bind ab168696 with a linear range of 0.156-1.25 µg/mL.
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SDS-PAGE - Recombinant human FGFR1 protein (Active) (ab168696)SDS-PAGE of reduced ab168696 stained overnight with Coomassie Blue. The protein migrates to 60-90 kDa under reducing conditions due to glycosylation.
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Functional Studies - Recombinant human FGFR1 protein (Active) (ab168696)Loaded ab168696 can bind ab155608 with an affinity constant of 0.16 uM as determined by BLI assay.
