Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
Overview
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Product name
Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control -
Specificity
ab170192, mouse IgG2b Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments. -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
General notes
This antibody clone is manufactured by Abcam.
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation. The control performed appropriately in all materials and platforms that were tested.
This product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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Purity
Ammonium Sulphate Precipitation -
Clonality
Monoclonal -
Clone number
7E10G10 -
Isotype
IgG2b -
Light chain type
kappa
Images
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Immunocytochemistry/ Immunofluorescence - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
Immunofluorscent analysis of NIH 3T3, COS7, H9C2 and HeLa cells using ab170192 at 1µg/ml (top); no primary antibody negative control (middle), and ab14705 as a positive control (bottom). An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
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Flow cytometry experiments with 4% PFA fixed Hela (human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast)were performed with the IgG2b Isotype Control Antibody (red) and no primary antibody negative control (black). An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody.
The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
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An isotyping ELISA was performed by coating a 96-well plate with 1.25 ug/mLab170192 and detecting with Alexa Fluor® conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG. This experiment verifies that the primary antibody's isotype is correct and that it is successfully bound by the secondary antibody.