HRP Anti-beta IV Tubulin antibody [ONS.1A6] (ab204454)
![HRP Anti-beta IV Tubulin antibody [ONS.1A6] (ab204454)](https://www.abcam.com/ps/products/204/ab204454/Images/ab204454-252322-anti-beta-iv-tubulin-antibody-ons1a6-hrp-western-blot.jpg "HRP Anti-beta IV Tubulin antibody [ONS.1A6] (ab204454)")
Key features and details
- HRP Mouse monoclonal [ONS.1A6] to beta IV Tubulin
- Suitable for: WB
- Reacts with: Mouse, Human
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-beta IV Tubulin antibody [ONS.1A6]
See all beta IV Tubulin primary antibodies -
Description
HRP Mouse monoclonal [ONS.1A6] to beta IV Tubulin -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide corresponding to beta IV Tubulin (C terminal) conjugated to bovine serum albumin.
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Positive control
- WB: HeLa, HEK293 and NIH3T3 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
ONS.1A6 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - HRP Anti-beta IV Tubulin antibody [ONS.1A6] (ab204454)All lanes : HRP Anti-beta IV Tubulin antibody [ONS.1A6] (ab204454) at 1/5000 dilution
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab204454 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
