Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CCR2 antibody [EPR20844-15] - BSA and Azide free (ab273061)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20844-15] to CCR2 - BSA and Azide free
  • Suitable for: ICC/IF, IP, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-CCR2 antibody [EPR20844-15] - BSA and Azide free
    See all CCR2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20844-15] to CCR2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, Flow Cytmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse spleen and lung tissue; Rat spleen tissue. ICC/IF: HEK-293T cells transfected with GFP tagged mouse CCR2 overexpression construct. Flow Cyt: HEK-293T cells transfected with GFP tagged mouse CCR2 overexpression construct. IP: Mouse lymph node tissue lysate.

  • General notes

    ab273061 is the carrier-free version of ab273050. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] - BSA and Azide free (ab273061)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] - BSA and Azide free (ab273061)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CCR2 with ab273050 at 1/250 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse spleen. The section was incubated with ab273050 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273050).

  • Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844-15] - BSA and Azide free (ab273061)
    Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844-15] - BSA and Azide free (ab273061)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) (transfected with GFP tagged mouse CCR2 overexpression construct) cells labelling CCR2 with ab273050 at 1/250 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (Red). Confocal image showing membranous and cytoplasmic staining in HEK-293T cells transfected with with GFP tagged mouse CCR2 overexpression construct. GFP is shown in Green. The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273050).

  • Flow Cytometry - Anti-CCR2 antibody [EPR20844-15] - BSA and Azide free (ab273061)
    Flow Cytometry - Anti-CCR2 antibody [EPR20844-15] - BSA and Azide free (ab273061)

    Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) (transfected with GFP tagged mouse CCR2 overexpression construct) cells labelling CCR2 with ab273050 at 1/600 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273050).

  • Immunoprecipitation - Anti-CCR2 antibody [EPR20844-15] - BSA and Azide free (ab273061)
    Immunoprecipitation - Anti-CCR2 antibody [EPR20844-15] - BSA and Azide free (ab273061)

    CCR2 was immunoprecipitated from 0.35 mg Mouse lymph node tissue lysate with ab273050 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273050 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.

    Lane 1: Mouse lymph node tissue lysate 10 ug.

    Lane 2: ab273050 IP in Mouse lymph node tissue lysate.

    Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab273050 in mouse lymph node tissue lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273050).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] - BSA and Azide free (ab273061)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] - BSA and Azide free (ab273061)

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CCR2 with ab273050 at 1/250 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat spleen. The section was incubated with ab273050 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273050).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] - BSA and Azide free (ab273061)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [EPR20844-15] - BSA and Azide free (ab273061)

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling CCR2 with ab273050 at 1/250 dilutionfollowed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse lung. The section was incubated with ab273050 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273050).

  • Anti-CCR2 antibody [EPR20844-15] - BSA and Azide free (ab273061)
    Anti-CCR2 antibody [EPR20844-15] - BSA and Azide free (ab273061)

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