Human soluble TREM1 ELISA Kit (ab185987)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 154 pg/ml
- Range: 500 pg/ml - 32000 pg/ml
- Sample type: Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human soluble TREM1 ELISA Kit
See all TREM1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 7.7% Inter-assay Sample n Mean SD CV% Overall 3 10.4% -
Sample type
Cell culture supernatant -
Assay type
Sandwich (quantitative) -
Sensitivity
154 pg/ml -
Range
500 pg/ml - 32000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 120 118% - 123% Cell culture media 90 85% - 100% Hep Plasma 115 113% - 118% EDTA Plasma 118 113% - 121% Cit plasma 110 105% - 115% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human soluble TREM1 SimpleStep ELISA® kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.This version will be discontinued when inventory is depleted. The new version is available as ab270884.
Human soluble TREM1 ELISA Kit (ab185987) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of soluble TREM1 protein in cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human soluble TREM1 with 154 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
The triggering receptor expressed on myeloid cells (TREM), modulate the innate response either by amplifying or dampening Toll-Like Receptor (TLR) induced signals, and act to fine-tuning the inflammatory response. Trem-1 is a member of the TREM family of receptors encoded by a cluster of genes localized to the Human chromosome 6p21.1. The presence of TREM and TREM-like receptors are expressed on a variety of innate cells of the myeloid lineage including neutrophils, monocytes, macrophages, microglia, dendritic cells, among others. Soluble Trem-1 is thought to arise through the cleavage of the receptor by metalloproteinases. Where it acts to negatively regulate TREM receptor signaling through neutralization of the respective ligands and minimize tissue damage due to prolonged inflammatory response.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human sTREM1 Capture Antibody 1 x 600µl 10X Human sTREM1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human sTREM1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
TREM1 is also known as triggering receptor expressed on myeloid cells 1, and triggering receptor expressed on monocytes 1. TREM1 is a Type I membrane protein that contains an immunoglobulin-like V-type domain. An alternatively spliced protein variant may be secreted. TREM1 is highly expressed on peripheral blood myeloid cells (particularly mature monocytes and granulocytes); TREM1 expression can be further upregulated by bacteria, fungi and lipopolysaccharide. TREM1 has been shown to interact with the adaptor protein DAP12 to stimulate neutrophil and monocyte-mediated inflammatory responses through the triggering and release of pro-inflammatory cytokines and chemokines. TREM1 is thought to amplify inflammatory responses to fungal and bacterial infections and potentiate septic shock. -
Cellular localization
Secreted and Cell membrane. -
Alternative names
- CD354
- TREM 1
- Triggering receptor expressed on monocytes 1
see all -
Database links
- Entrez Gene: 54210 Human
- Omim: 605085 Human
- SwissProt: Q9NP99 Human
Images
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Other - Human soluble TREM1 ELISA Kit (ab185987)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of sTREM1 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of sTREM1 standard curve in Sample Diluent 25BS.Background-subtracted data values (mean +/- SD) are graphed.
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Titration of HL60 Conditioned Cell Culture Media diluted to within the working range of the assay.HL60 cells were grown in culture with or without the addition of (5 µg/mL) LPS for 24 hours before supernatants were collected and assayed. Background subtracted data from duplicate measurements are plotted.
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Titration of HL60 Conditioned Cell Culture Media diluted to within the working range of the assay.HL60 cells were grown in culture with or without the addition of (5 µg/mL) LPS for 24 hours before supernatants were collected and assayed. Bar graph denotes quantification of sTREM1 interpolated from standard curve and multiplied by dilution factor.
