Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GBP1 antibody [EPR8285] - BSA and Azide free (ab240050)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8285] to GBP1 - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-GBP1 antibody [EPR8285] - BSA and Azide free
    See all GBP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8285] to GBP1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549 and HeLa (Untreated or treated with 10ng/ml IFN-? for 24 hours) cell lysates. ICC/IF: IFN-? induced HeLa cells. IHC-P: Human spleen tissue.

  • General notes

    ab240050 is the carrier-free version of ab131255 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab240050 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GBP1 antibody [EPR8285] - BSA and Azide free (ab240050)
    Western blot - Anti-GBP1 antibody [EPR8285] - BSA and Azide free (ab240050)
    All lanes : Anti-GBP1 antibody [EPR8285] (ab131255) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : GBP1 knockout A549 cell lysate
    Lane 3 : HeLa treated with 10ng/ml IFN-? for 24 hours, whole cell lysate
    Lane 4 : Untreated HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 68 kDa
    Observed band size: 68 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab131255).

    Lanes 1-4: Merged signal (red and green). Green - ab131255 observed at 68 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab131255 Anti-GBP1 antibody [EPR8285] was shown to specifically react with GBP1 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267202 (knockout cell lysate ab257960) was used. Wild-type and GBP1 knockout samples were subjected to SDS-PAGE. ab131255 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-GBP1 antibody [EPR8285] - BSA and Azide free (ab240050)
    Immunocytochemistry/ Immunofluorescence - Anti-GBP1 antibody [EPR8285] - BSA and Azide free (ab240050)
    Immunofluorescent analysis of IFN-gamma induced HeLa cells labelling GBP1 with ab131255 antibody at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131255).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP1 antibody [EPR8285] - BSA and Azide free (ab240050)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP1 antibody [EPR8285] - BSA and Azide free (ab240050)

    Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling GBP1 with ab131255 antibody at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131255).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-GBP1 antibody [EPR8285] - BSA and Azide free (ab240050)
    OI-RD Scanning - Anti-GBP1 antibody [EPR8285] - BSA and Azide free (ab240050)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131255).

  • Anti-GBP1 antibody [EPR8285] - BSA and Azide free (ab240050)
    Anti-GBP1 antibody [EPR8285] - BSA and Azide free (ab240050)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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