Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GBP1 antibody [EPR8285] (ab131255)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8285] to GBP1
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-GBP1 antibody [EPR8285]
    See all GBP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8285] to GBP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human GBP1 aa 550-650. The exact sequence is proprietary.

  • Positive control

    • WB: A549 and HeLa (with and without treatment with IFN gamma) cell lysates. IHC-P: Human spleen tissue. ICC/IF: IFN gamma induced HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GBP1 antibody [EPR8285] (ab131255)
    Western blot - Anti-GBP1 antibody [EPR8285] (ab131255)
    All lanes : Anti-GBP1 antibody [EPR8285] (ab131255) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : GBP1 knockout A549 cell lysate
    Lane 3 : HeLa treated with 10ng/ml IFN-? for 24 hours, whole cell lysate
    Lane 4 : Untreated HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 68 kDa
    Observed band size: 68 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab131255 observed at 68 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab131255 Anti-GBP1 antibody [EPR8285] was shown to specifically react with GBP1 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267202 (knockout cell lysate ab257960) was used. Wild-type and GBP1 knockout samples were subjected to SDS-PAGE. ab131255 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-GBP1 antibody [EPR8285] (ab131255)
    Immunocytochemistry/ Immunofluorescence - Anti-GBP1 antibody [EPR8285] (ab131255)
    Immunofluorescent analysis of IFN-gamma induced HeLa cells labelling GBP1 with ab131255 antibody at 1/100 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP1 antibody [EPR8285] (ab131255)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP1 antibody [EPR8285] (ab131255)

    Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling GBP1 with ab131255 antibody at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-GBP1 antibody [EPR8285] (ab131255)
    Western blot - Anti-GBP1 antibody [EPR8285] (ab131255)
    All lanes : Anti-GBP1 antibody [EPR8285] (ab131255) at 1/1000 dilution

    Lane 1 : HeLa cell lysate, treated with IFN gamma
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

    Predicted band size: 68 kDa

  • OI-RD Scanning - Anti-GBP1 antibody [EPR8285] (ab131255)
    OI-RD Scanning - Anti-GBP1 antibody [EPR8285] (ab131255)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-GBP1 antibody [EPR8285] (ab131255)
    Anti-GBP1 antibody [EPR8285] (ab131255)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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