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Anti-GBP1 antibody [OTI1B2] (ab119236)

Key features and details

  • Mouse monoclonal [OTI1B2] to GBP1
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-GBP1 antibody [OTI1B2]
    See all GBP1 primary antibodies

  • Description

    Mouse monoclonal [OTI1B2] to GBP1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    African green monkey
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human GBP1 aa 1 to the C-terminus. Produced in HEK-293T cells (NP_002044).
    Database link: P32455

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HEK-293T cell lysate transfected with pCMV6-ENTRY GBP1 cDNA, Mouse spleen extract; Human testis, omentum, uterus, liver, ovary, thyroid gland, colon and spleen extracts. IHC-P: Human lung carcinoma tissue. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY GBP1 cDNA. Flow Cyt: HEK-293T cells transfected with pCMV6-ENTRY GBP1 cDNA.

  • General notes

    Clone OTI1B2 (formerly 1B2).

Properties

Images

  • Western blot - Anti-GBP1 antibody [OTI1B2] (ab119236)
    Western blot - Anti-GBP1 antibody [OTI1B2] (ab119236)
    All lanes : Anti-GBP1 antibody [OTI1B2] (ab119236) at 1/2000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY GBP1 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 68 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP1 antibody [OTI1B2] (ab119236)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP1 antibody [OTI1B2] (ab119236)

    Paraffin-embedded human lung carcinoma tissue stained for GBP1 using ab119236 at 1/150 in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-GBP1 antibody [OTI1B2] (ab119236)
    Immunocytochemistry/ Immunofluorescence - Anti-GBP1 antibody [OTI1B2] (ab119236)

    COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY GBP1 cDNA, stained for GBP1 using ab119236 at 1/100 in ICC/IF.

  • Western blot - Anti-GBP1 antibody [OTI1B2] (ab119236)
    Western blot - Anti-GBP1 antibody [OTI1B2] (ab119236)
    All lanes : Anti-GBP1 antibody [OTI1B2] (ab119236) at 1/200 dilution

    Lane 1 : Human testis extract
    Lane 2 : Human omentum extract
    Lane 3 : Human uterus extract
    Lane 4 : Human breast extract
    Lane 5 : Human brain extract
    Lane 6 : Human liver extract
    Lane 7 : Human ovary extract
    Lane 8 : Human thyroid gland extract
    Lane 9 : Human colon extract
    Lane 10 : Human spleen extract

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 68 kDa

  • Flow Cytometry - Anti-GBP1 antibody [OTI1B2] (ab119236)
    Flow Cytometry - Anti-GBP1 antibody [OTI1B2] (ab119236)

    Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY GBP1 (red) or empty vector control plasmid (blue) labeling GBP1 with ab119236 at 1/100 dilution.

  • Western blot - Anti-GBP1 antibody [OTI1B2] (ab119236)
    Western blot - Anti-GBP1 antibody [OTI1B2] (ab119236)
    Anti-GBP1 antibody [OTI1B2] (ab119236) at 1/200 dilution + Mouse spleen extract at 10 µg

    Predicted band size: 68 kDa

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