Antibodies, Proteins, Kits and Reagents for Life Science

308 236 ₸ Product size

100 µg 308 236 ₸

Order now and get it on Tuesday March 09, 2021

Anti-TLR9 antibody [26C593.2] (ab134368)

Key features and details

Mouse monoclonal [26C593.2] to TLR9
Suitable for: WB, IHC-Fr, Flow Cyt, ICC/IF, IHC-P
Reacts with: Mouse, Rat, Human
Isotype: IgG1

Product name

Anti-TLR9 antibody [26C593.2]
See all TLR9 primary antibodies
Description

Mouse monoclonal [26C593.2] to TLR9
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-Fr	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human TLR9 aa 268-284 conjugated to keyhole limpet haemocyanin. AAF78037
Sequence:
CPRHFPQLHPDTFSHLS

Database link: Q9NR96
Run BLAST with Run BLAST with
Positive control
- WB: Human peripheral blood mononuclear cells (PBMCs). Human, mouse and rat intestine tissue lysates. Flow Cytometry: Ramos and PBMC cells. IHC-Fr: Human lung adenocarcinoma and malignant lung tissues. IHC-P: Human spleen tissue. ICC/IF: A549 cells.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at 4°C (up to 6 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS
Concentration information loading...
Purity

Protein G purified
Clonality

Monoclonal
Clone number

26C593.2
Isotype

IgG1
Light chain type

kappa
Research areas

Flow Cytometry - Anti-TLR9 antibody [26C593.2] (ab134368)

Flow Cytometric analysis of TLR9 in Ramos (Human Burkitt's lymphoma cell line) cells labelled with ab134368 at 0.1 µg (pink), no antibody (blue) or isotype control antibody (green). Goat anti-mouse IgG FITC secondary was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody [26C593.2] (ab134368)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling TLR9 with ab134368 at 5 µg/ml.

Staining was enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Western blot - Anti-TLR9 antibody [26C593.2] (ab134368)

All lanes : Anti-TLR9 antibody [26C593.2] (ab134368) at 3 µg/ml

Lane 1 : Human peripheral blood mononuclear cells (PBMCs)
Lane 2 : Human intestine tissue lysate
Lane 3 : Mouse intestine tissue lysate
Lane 4 : Rat intestine tissue lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : Goat anti-mouse HRP conjugate

Predicted band size: 115 kDa
Observed band size: 120 kDa
why is the actual band size different from the predicted?
Flow Cytometry - Anti-TLR9 antibody [26C593.2] (ab134368)

Flow Cytometric analysis of TLR9 in human PBMC cells labelled with ab134368 at 0.5 µg (red) or an isotype control antibody (green).
Immunohistochemistry (Frozen sections) - Anti-TLR9 antibody [26C593.2] (ab134368)

Immunohistochemical analysis of TLR9 in frozen sections of human lung adenocarcinoma tissue, using ab134368 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-TLR9 antibody [26C593.2] (ab134368)

Immunohistochemical analysis of TLR9 in frozen sections of A549 (Human lung carcinoma cell line) cells, using ab134368 at a 1/100 dilution.
Immunohistochemistry (Frozen sections) - Anti-TLR9 antibody [26C593.2] (ab134368)

Immunohistochemical analysis of TLR9 in frozen sections of malignant human lung tissue, using ab134368 at a 1/100 dilution.

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