Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-10RB/IL-10R2 antibody [EPR24691-11] - BSA and Azide free (ab280572)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24691-11] to IL-10RB/IL-10R2 - BSA and Azide free
  • Suitable for: Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-IL-10RB/IL-10R2 antibody [EPR24691-11] - BSA and Azide free
    See all IL-10RB/IL-10R2 primary antibodies

  • Description

    Rabbit monoclonal [EPR24691-11] to IL-10RB/IL-10R2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cytmore details
    Unsuitable for: ICC,IHC-P or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: Human PBMC cells.

  • General notes

    ab280572 is the carrier-free version of ab273038. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280572 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-IL-10RB/IL-10R2 antibody [EPR24691-11] - BSA and Azide free (ab280572)
    Flow Cytometry - Anti-IL-10RB/IL-10R2 antibody [EPR24691-11] - BSA and Azide free (ab280572)

    This data was developed using ab273038, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling IL-10RB/IL-10R2 with ab273038 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab273038. Then stained with anti-CD14 conjugated to BV510. Gated on viable cells.

  • Flow Cytometry - Anti-IL-10RB/IL-10R2 antibody [EPR24691-11] - BSA and Azide free (ab280572)
    Flow Cytometry - Anti-IL-10RB/IL-10R2 antibody [EPR24691-11] - BSA and Azide free (ab280572)

    This data was developed using ab273038, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling IL-10RB/IL-10R2 with ab273038 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab273038. Then stained with anti-CD3 conjugated to Alexa Fluor® 647. Gated on viable cells.

  • Flow Cytometry - Anti-IL-10RB/IL-10R2 antibody [EPR24691-11] - BSA and Azide free (ab280572)
    Flow Cytometry - Anti-IL-10RB/IL-10R2 antibody [EPR24691-11] - BSA and Azide free (ab280572)

    This data was developed using ab273038, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling IL-10RB/IL-10R2 with ab273038 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab273038. Then stained with anti-CD3 conjugated to Alexa Fluor® 647 and anti-CD14 conjugated to BV510. Gated on viable T cells and monocytes.

  • Flow Cytometry - Anti-IL-10RB/IL-10R2 antibody [EPR24691-11] - BSA and Azide free (ab280572)
    Flow Cytometry - Anti-IL-10RB/IL-10R2 antibody [EPR24691-11] - BSA and Azide free (ab280572)

    This data was developed using ab273038, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling IL-10RB/IL-10R2 with ab273038 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab273038. Then stained with anti-CD56 conjugated to PE. Gated on viable NK cells.

  • Anti-IL-10RB/IL-10R2 antibody [EPR24691-11] - BSA and Azide free (ab280572)
    Anti-IL-10RB/IL-10R2 antibody [EPR24691-11] - BSA and Azide free (ab280572)

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