Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TLR9 antibody [EPR21735] - BSA and Azide free (ab232933)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21735] to TLR9 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-TLR9 antibody [EPR21735] - BSA and Azide free
    See all TLR9 primary antibodies

  • Description

    Rabbit monoclonal [EPR21735] to TLR9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Raji whole cell lysate.

  • General notes

    Ab232933 is the carrier-free version of ab211012. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232933 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-TLR9 antibody [EPR21735] - BSA and Azide free (ab232933)
    Immunoprecipitation - Anti-TLR9 antibody [EPR21735] - BSA and Azide free (ab232933)

    TLR9 was immunoprecipitated from 0.35 mg of Raji (human Burkitt's lymphoma cell line) whole cell lysate with ab211012 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211012 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Raji whole cell lysate 10 µg (Input). 

    Lane 2: ab211012 IP in Raji whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211012 in Raji whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    The cleaved TLR9 (60 kDa) observed is consistent with the literature (PMID: 24582318).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211012).

  • Western blot - Anti-TLR9 antibody [EPR21735] - BSA and Azide free (ab232933)
    Western blot - Anti-TLR9 antibody [EPR21735] - BSA and Azide free (ab232933)
    All lanes : Anti-TLR9 antibody [EPR21735] (ab211012) at 1/1000 dilution

    Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell lysate at 10 µg
    Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 116 kDa
    Observed band size: 120,60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The cleaved TLR9 (60 kDa) observed is consistent with the literature (PMID: 24582318).

    Untreated THP1s have undetectable TLR9, as described in the literature (PMID: 20375564).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211012).

  • Anti-TLR9 antibody [EPR21735] - BSA and Azide free (ab232933)
    Anti-TLR9 antibody [EPR21735] - BSA and Azide free (ab232933)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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