Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Immunology Innate Immunity Macrophage / Inflamm.

Anti-TLR9 antibody [EPR21735] (ab211012)

Price and availability

291 484 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-TLR9 antibody [EPR21735] (ab211012)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21735] to TLR9
  • Suitable for: WB, IP
  • Reacts with: Human

You may also be interested in

TLR7 peptide (ab261647)
Product image
Recombinant Mouse TARC/CCL17 protein (ab276756)
Product image
Anti-C Reactive Protein antibody - BSA and Azide free (Capture) (ab259733)
Product image
Anti-Macrophage antibody [RM0029-11H3] (ab56297)

Overview

  • Product name

    Anti-TLR9 antibody [EPR21735]
    See all TLR9 primary antibodies
  • Description

    Rabbit monoclonal [EPR21735] to TLR9
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Raji and Daudi whole cell lysates. IP: Raji whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21735
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Immunoglobulins
    • Receptors
    • Signal Transduction
    • Adapters
    • Transmembrane
    • Immunology
    • Innate Immunity
    • TLR Signaling

Images

  • Western blot - Anti-TLR9 antibody [EPR21735] (ab211012)
    Western blot - Anti-TLR9 antibody [EPR21735] (ab211012)
    All lanes : Anti-TLR9 antibody [EPR21735] (ab211012) at 1/1000 dilution

    Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell lysate at 10 µg
    Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 116 kDa
    Observed band size: 120,60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The cleaved TLR9 (60 kDa) observed is consistent with the literature (PMID: 24582318).

    Untreated THP1s have undetectable TLR9, as described in the literature (PMID: 20375564).

  • Western blot - Anti-TLR9 antibody [EPR21735] (ab211012)
    Western blot - Anti-TLR9 antibody [EPR21735] (ab211012)
    Anti-TLR9 antibody [EPR21735] (ab211012) at 1/1000 dilution + Daudi (human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 116 kDa
    Observed band size: 120,60 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The cleaved TLR9 (60 kDa) observed is consistent with the literature (PMID: 24582318).

  • Immunoprecipitation - Anti-TLR9 antibody [EPR21735] (ab211012)
    Immunoprecipitation - Anti-TLR9 antibody [EPR21735] (ab211012)

    TLR9 was immunoprecipitated from 0.35 mg of Raji (human Burkitt's lymphoma cell line) whole cell lysate with ab211012 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211012 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Raji whole cell lysate 10 µg (Input). 

    Lane 2: ab211012 IP in Raji whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211012 in Raji whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    The cleaved TLR9 (60 kDa) observed is consistent with the literature (PMID: 24582318).

  • Anti-TLR9 antibody [EPR21735] (ab211012)
    Anti-TLR9 antibody [EPR21735] (ab211012)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-TLR9 antibody [EPR21735] (ab211012)

  •  
  • Product image

    Anti-TLR9 antibody [EPR14964-2] (ab187148)

    Applications: WB

  •  
  • Product image

    FITC Anti-TLR9 antibody [5G5] (ab58864)

    Applications: Flow Cyt

  •  
  • Product image

    FITC Anti-TLR9 antibody [ABM4D70] (ab210925)

    Applications: Flow Cyt

  •  
  • Product image

    Anti-TLR9 antibody (ab37154)

    Applications: ICC/IF, IHC-P, WB

  •  
  • Product image

    Anti-TLR9 antibody [26C593.2] (ab134368)

    Applications: Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB

  •  
  • Product image

    Anti-TLR9 antibody (ab125139)

    Applications: IHC-P, WB

  •  
  • Product image

    Anti-TLR9 antibody (ab52967)

    Applications: ICC, WB

  •  
  • Product image

    Anti-TLR9 antibody (ab62577)

    Applications: ICC, ICC/IF, WB

  •  
  • Product image

    Anti-TLR9 antibody - N-terminal (ab227004)

    Applications: IHC-P, WB

Clear all

Recently viewed products

  •  
  • Product image

    Anti-CD20 antibody (ab27093)

  •  
  • Product image

    Anti-SEMA6B antibody (ab204750)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.