Anti-TLR9 antibody [EPR21735] (ab211012)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21735] to TLR9
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-TLR9 antibody [EPR21735]
See all TLR9 primary antibodies -
Description
Rabbit monoclonal [EPR21735] to TLR9 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Raji and Daudi whole cell lysates. IP: Raji whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21735 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-TLR9 antibody [EPR21735] (ab211012) at 1/1000 dilution
Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell lysate at 10 µg
Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 116 kDa
Observed band size: 120,60 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The cleaved TLR9 (60 kDa) observed is consistent with the literature (PMID: 24582318).
Untreated THP1s have undetectable TLR9, as described in the literature (PMID: 20375564).
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Anti-TLR9 antibody [EPR21735] (ab211012) at 1/1000 dilution + Daudi (human Burkitt's lymphoma cell line) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 116 kDa
Observed band size: 120,60 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The cleaved TLR9 (60 kDa) observed is consistent with the literature (PMID: 24582318).
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TLR9 was immunoprecipitated from 0.35 mg of Raji (human Burkitt's lymphoma cell line) whole cell lysate with ab211012 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211012 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Raji whole cell lysate 10 µg (Input).
Lane 2: ab211012 IP in Raji whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211012 in Raji whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
The cleaved TLR9 (60 kDa) observed is consistent with the literature (PMID: 24582318).
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