Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SMG5 antibody [EPR3503(2)] (ab129107)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3503(2)] to SMG5
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-SMG5 antibody [EPR3503(2)]
    See all SMG5 primary antibodies

  • Description

    Rabbit monoclonal [EPR3503(2)] to SMG5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human SMG5 aa 950-1050. The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, and K562 cell lysates; Human breast carcinoma and thyroid gland carcinoma tissues; and 293T cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR3503(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-SMG5 antibody [EPR3503(2)] (ab129107)
    Western blot - Anti-SMG5 antibody [EPR3503(2)] (ab129107)
    All lanes : Anti-SMG5 antibody [EPR3503(2)] (ab129107) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 114 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMG5 antibody [EPR3503(2)] (ab129107)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMG5 antibody [EPR3503(2)] (ab129107)

    ab129107, at 1/100 dilution, staining SMG5 in Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMG5 antibody [EPR3503(2)] (ab129107)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMG5 antibody [EPR3503(2)] (ab129107)

    ab129107, at 1/100 dilution, staining SMG5 in Paraffin-embedded Human thyroid gland carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SMG5 antibody [EPR3503(2)] (ab129107)
    Immunocytochemistry/ Immunofluorescence - Anti-SMG5 antibody [EPR3503(2)] (ab129107)
    ab129107, at 1/100 dilution, staining SMG5 in 293T cells by Immunofluorescence.
  • Anti-SMG5 antibody [EPR3503(2)] (ab129107)
    Anti-SMG5 antibody [EPR3503(2)] (ab129107)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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