Anti-SMG5 antibody (ab33033)
Key features and details
- Rabbit polyclonal to SMG5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SMG5 antibody
See all SMG5 primary antibodies -
Description
Rabbit polyclonal to SMG5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1000 to the C-terminus of Human SMG5.
Read Abcam's proprietary immunogen policy (Peptide available as ab36628.) -
Positive control
- Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab33033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-P HumanWB HumanAll applications MouseApplication Abreviews Notes WB 1/250. Detects a band of approximately 140 kDa (predicted molecular weight: 114 kDa).IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.Notes WB
1/250. Detects a band of approximately 140 kDa (predicted molecular weight: 114 kDa).IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.Application notesIs unsuitable for ICC/IF.Target
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Relevance
SMG5 plays a role in nonsense mediated mRNA decay. It promotes dephosphorylation of RENT1, and is necessary for TERT activity. It is part of a complex that contains SMG1, SMG5, SMG7, PP2AC, a short isoform of UPF3A (isoform UPF3AS, but not isoform UPF3AL) and phosphorylated RENT1/UPF1. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 23381 Human
- Entrez Gene: 229512 Mouse
- Omim: 610962 Human
- SwissProt: Q9UPR3 Human
- SwissProt: Q6ZPY2 Mouse
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Alternative names
- EST1 like protein B antibody
- EST1B antibody
- LPTS interacting protein antibody
see all
Images
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Western blot - Anti-SMG5 antibody (ab33033)Anti-SMG5 antibody (ab33033) at 1/250 dilution +
Jurkat whole cell lysate (ab7899) at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 114 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?The band seen at 140 kDa is consistent with the banding pattern observed for other commercially available antibodies to SMG5.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMG5 antibody (ab33033)IHC image of ab33033 staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab33033, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
References (2)
ab33033 has been referenced in 2 publications.
- Wada M et al. Interplay between coronavirus, a cytoplasmic RNA virus, and nonsense-mediated mRNA decay pathway. Proc Natl Acad Sci U S A 115:E10157-E10166 (2018). PubMed: 30297408
- Park OH et al. Identification and molecular characterization of cellular factors required for glucocorticoid receptor-mediated mRNA decay. Genes Dev 30:2093-2105 (2016). PubMed: 27798850
Images
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Western blot - Anti-SMG5 antibody (ab33033)Anti-SMG5 antibody (ab33033) at 1/250 dilution +
Jurkat whole cell lysate (ab7899) at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 114 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?The band seen at 140 kDa is consistent with the banding pattern observed for other commercially available antibodies to SMG5.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMG5 antibody (ab33033)IHC image of ab33033 staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab33033, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
