Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-NDUFB11 antibody [EPR15252] (ab183716)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15252] to NDUFB11
Suitable for: WB, Flow Cyt, IHC-P
Reacts with: Human

Product name

Anti-NDUFB11 antibody [EPR15252]
See all NDUFB11 primary antibodies
Description

Rabbit monoclonal [EPR15252] to NDUFB11
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human heart lysate; HeLa, HepG2 and A431 cell lysates; Human kidney tissue; HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15252
Isotype

IgG
Research areas

Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716)

Anti-NDUFB11 antibody [EPR15252] (ab183716) at 1/10000 dilution + Human heart lysate at 20 µg

Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 17 kDa
Observed band size: 17 kDa
Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716)

Lanes 1-2 : Anti-NDUFB11 antibody [EPR15252] (ab183716) at 1/50000 dilution
Lane 3 : Anti-NDUFB11 antibody [EPR15252] (ab183716) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A431 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 17 kDa
Observed band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB11 antibody [EPR15252] (ab183716)

Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling NDUFB11 with ab183716 at a 1/250 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-NDUFB11 antibody [EPR15252] (ab183716)

Flow cytometry analysis of paraformaldehyde-fixed HeLa cells labeling NDUFB11 with ab183716 at a 1/240 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).
Anti-NDUFB11 antibody [EPR15252] (ab183716)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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