Abcam’s Thyroglobulin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human Thyroglobulin in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human Thyroglobulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Thyroglobulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Thyroglobulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Thyroglobulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.