Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Wednesday March 10, 2021

Anti-MICA + MICB antibody [EPR22031] (ab224702)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22031] to MICA + MICB
Suitable for: WB, IHC-P
Reacts with: Human, Recombinant fragment

Product name

Anti-MICA + MICB antibody [EPR22031]
See all MICA + MICB primary antibodies
Description

Rabbit monoclonal [EPR22031] to MICA + MICB
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

Recombinant fragment

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: His-tagged MICB recombinant protein expressed by the E. coli system (aa23-383 with 310-330 replaced by GGGS). His-tagged MICA recombinant protein expressed by the E. coli system (aa24-383 with 308-328 replaced by GGGGS). PNGase F treated HeLa and K562 cell lysate. U266B1, HUVEC, K562 and HeLa whole cell lysate. IHC-P: Human ovary cancer, lung cancer and stomach tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human Recombinant fragment

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22031
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICA + MICB antibody [EPR22031] (ab224702)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling MICA with ab224702 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on epithelium of human stomach (PMID: 28334733) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-MICA + MICB antibody [EPR22031] (ab224702)

All lanes : Anti-MICA + MICB antibody [EPR22031] (ab224702) at 1/1000 dilution

Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow), whole cell lysate
Lane 2 : K562 whole cell lysate treated with PNGase F
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate
Lane 4 : HeLa whole cell lysate treated with PNGase F
Lane 5 : HUVEC (human umbilical vein endothelial cell line), whole cell lysate
Lane 6 : U266B1 (human multiple myeloma B lymphocyte cell line), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 42 kDa
Observed band size: 40,50-60 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Bands between 50-60kDa represent the glycosylated form. After PNGase F treatment, the de-glycosylated form appears around 40kDa.

The molecular weight observed is consistent with what has been described in the literature (PMID: 10359807; PMID: 9396860).

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICA + MICB antibody [EPR22031] (ab224702)

Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue labeling MICA with ab224702 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on human ovary cancer (PMID: 28334733) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICA + MICB antibody [EPR22031] (ab224702)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling MICA with ab224702 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on human lung cancer (PMID: 28334733) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-MICA + MICB antibody [EPR22031] (ab224702)

All lanes : Anti-MICA + MICB antibody [EPR22031] (ab224702) at 1/5000 dilution

Lane 1 : His-tagged MICB recombinant protein expressed in E.coli system (aa23-383 with 310-330 replaced by GGGS), 10 ng
Lane 2 : His-tagged MICA recombinant protein expressed in E.coli system (aa24-383 with 308-328 replaced by GGGGS) 10 ng

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 42 kDa

Exposure time: 15 seconds

Blocking and dilution buffer: 5% NFDM/TBST.
Anti-MICA + MICB antibody [EPR22031] (ab224702)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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