Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MICA + MICB antibody [EPR22031] (ab224702)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22031] to MICA + MICB
  • Suitable for: WB, IHC-P
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-MICA + MICB antibody [EPR22031]
    See all MICA + MICB primary antibodies

  • Description

    Rabbit monoclonal [EPR22031] to MICA + MICB

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged MICB recombinant protein expressed by the E. coli system (aa23-383 with 310-330 replaced by GGGS). His-tagged MICA recombinant protein expressed by the E. coli system (aa24-383 with 308-328 replaced by GGGGS). PNGase F treated HeLa and K562 cell lysate. U266B1, HUVEC, K562 and HeLa whole cell lysate. IHC-P: Human ovary cancer, lung cancer and stomach tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22031

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICA + MICB antibody [EPR22031] (ab224702)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICA + MICB antibody [EPR22031] (ab224702)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling MICA with ab224702 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on epithelium of human stomach (PMID: 28334733) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-MICA + MICB antibody [EPR22031] (ab224702)
    Western blot - Anti-MICA + MICB antibody [EPR22031] (ab224702)
    All lanes : Anti-MICA + MICB antibody [EPR22031] (ab224702) at 1/1000 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow), whole cell lysate
    Lane 2 : K562 whole cell lysate treated with PNGase F
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate
    Lane 4 : HeLa whole cell lysate treated with PNGase F
    Lane 5 : HUVEC (human umbilical vein endothelial cell line), whole cell lysate
    Lane 6 : U266B1 (human multiple myeloma B lymphocyte cell line), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 42 kDa
    Observed band size: 40,50-60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Bands between 50-60kDa represent the glycosylated form. After PNGase F treatment, the de-glycosylated form appears around 40kDa.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 10359807; PMID: 9396860).

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICA + MICB antibody [EPR22031] (ab224702)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICA + MICB antibody [EPR22031] (ab224702)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue labeling MICA with ab224702 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on human ovary cancer (PMID: 28334733) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICA + MICB antibody [EPR22031] (ab224702)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICA + MICB antibody [EPR22031] (ab224702)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling MICA with ab224702 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on human lung cancer (PMID: 28334733) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-MICA + MICB antibody [EPR22031] (ab224702)
    Western blot - Anti-MICA + MICB antibody [EPR22031] (ab224702)
    All lanes : Anti-MICA + MICB antibody [EPR22031] (ab224702) at 1/5000 dilution

    Lane 1 : His-tagged MICB recombinant protein expressed in E.coli system (aa23-383 with 310-330 replaced by GGGS), 10 ng
    Lane 2 : His-tagged MICA recombinant protein expressed in E.coli system (aa24-383 with 308-328 replaced by GGGGS) 10 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 42 kDa


    Exposure time: 15 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-MICA + MICB antibody [EPR22031] (ab224702)
    Anti-MICA + MICB antibody [EPR22031] (ab224702)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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