Anti-HLA-C antibody (ab232903)
Key features and details
- Rabbit polyclonal to HLA-C
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-HLA-C antibody
See all HLA-C primary antibodies -
Description
Rabbit polyclonal to HLA-C -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human HLA-C aa 1-350. (Expressed in E.coli).
Database link: P10321 -
Positive control
- WB: Recombinant human HLA-C protein; Human lymphocyte lysate; A431 cell lysate; Pig liver lysate. IHC-P: Human lung cancer, liver, kidney, prostate, skin cancer and stomach tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab232903 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-HLA-C antibody (ab232903) at 2 µg/ml + Recombinant human HLA-C protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 41 kDa
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All lanes : Anti-HLA-C antibody (ab232903) at 2 µg/ml
Lane 1 : Human lymphocyte lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 3 : Pig liver lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 41 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody (ab232903)
Paraffin-embedded human lung cancer tissue stained for HLA-C using ab232903 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody (ab232903)
Paraffin-embedded human liver tissue stained for HLA-C using ab232903 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody (ab232903)
Paraffin-embedded human kidney tissue stained for HLA-C using ab232903 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody (ab232903)
Paraffin-embedded human prostate tissue stained for HLA-C using ab232903 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody (ab232903)
Paraffin-embedded human skin cancer tissue stained for HLA-C using ab232903 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody (ab232903)
Paraffin-embedded human stomach tissue stained for HLA-C using ab232903 at 20 μg/ml in immunohistochemical analysis. DAB staining.